水稻白叶枯病菌udgH基因的功能研究  

Functional Analysis of a Annotated udg H Gene in Xanthomona oryzae pv.oryzae

作　　者：黄胜[1,2] 林茵[1,2] 倪哲[1,2] 杨文珊 姜伟[1,2] 何勇强[1,2] 周行[3,2] 

机构地区：[1]广西大学生命科学与技术学院,南宁530004 [2]微生物及植物遗传工程教育部重点实验室广西大学,南宁530004 [3]广西农业科学院,南宁530007

出　　处：《基因组学与应用生物学》2015年第6期1218-1224,共7页Genomics and Applied Biology

基　　金：农业部行业专项(201303015);微生物及植物遗传工程教育部重点实验室开放课题(J1301);广西大学科研基金资助项目(XGZ130088);广西科技攻关项目(桂科合13999001;桂科能1347006-1)共同资助

摘　　要：水稻白叶枯病菌(Xanthomonas oryzae pv.oryzae,以下简称Xoo)是水稻病原菌的一种,其引起的水稻白叶枯病(bacterial leaf blight,BLB)可致使水稻大量减产。本研究发现一个假定的udg H基因的插入突变致使致病力急剧下降,并且胞外多糖、抗逆性、生物膜、游动性等几种生理表型与野生型相比发生了明显变化。互补菌株的致病力能恢复至80%以上,其他生理表型基本能恢复至野生型。本研究为进一步研究udg H基因的致病分子机理提供了研究线索。Bacterial leaf blight(BLB) caused by gram-negative bacterium Xanthomonas oryzae pv. oryzae(Xoo) is a devastating rice disease in the world. In our preliminary study, a random Tn5 transposon-mediated mutation library of Guangxi Xoo strain K74 was generated. Here, we characterized an udg H mutant of Xoo K74 and showed that the udg H mutant resulted in a reduction in virulence on the host. We also demonstrated that udg H is involved in cell motility, biofilm formation ability and polysaccharide synthesis. Furthermore, the udg H mutant showed increased sensitivity to hydrogen peroxide and SDS. All these phenotype in complementary strain can be restored to wild-type levels but virulence only around 80%. This work laid a solid foundation for the further study on pathogenic molecular mechanism of udg H.

关 键 词：水稻白叶枯病菌 UDP-脱氢酶 致病力 

分 类 号：S435.111.47[农业科学—农业昆虫与害虫防治]