巨桉叶片总RNA提取方法比较  

Comparison on Methods for Total RNA Extraction from E. grandis Leaves

作　　者：杨婕[1,2] 郭文硕[1,2] 叶小真[1] 谢婉凤[1] 张思庭 黄爱珍[2] 

机构地区：[1]福建农林大学森林保护研究所,福州350002 [2]福建农林大学林学院,福州350002

出　　处：《基因组学与应用生物学》2015年第6期1272-1276,共5页Genomics and Applied Biology

基　　金：桉树病害防治技术研究与推广(福建省财政厅社会公益研究)项目(K8112004A)资助

摘　　要：本研究以巨桉嫩叶为材料,通过试剂盒法、改良CTAB法和改良SDS法,进行巨桉嫩叶总RNA提取方法的比较试验,探讨适合巨桉嫩叶总RNA提取的方法。通过超微量分光光度计、凝胶电泳和RT-PCR对总RNA提取质量进行检验,结果表明:改良CTAB和改良SDS法均能获得高质量的总RNA,条带清晰,纯度高,效果稳定,均适用于桉树叶片总RNA提取。Young leaves of E.grandis were used for isolation of total RNA by Trizol methods, modified CTAB,modified SDS. The quality of total RNA were determined by gel electrophoresis, ultramicro spectrophotometer and RT-PCR. The results indicated that modified CTAB and modified SDS could isolate higher quanlity RNA from E. grandis leaves. The total RNA obtained by modified SDS method was highly integrate and pure with clear28 S and 18 S bands.This method could be used for total RNA extraction from E. grandis leaves.

关 键 词：巨桉 总RNA 试剂盒法 改良CTAB法 改良SDS法 

分 类 号：S792.39[农业科学—林木遗传育种]