普通野生稻染色体片段代换系的孕穗期耐冷性研究  

Study on Cold Tolerance of Chromosome Segment Substitution Lines Derived from Wild Rice(O. rufipogon) as Donor at Booting Stage

作　　者：覃宝祥[1,2] 刘立龙[1,2] 韩飞怡 陈霖 李楠 黄敏[3] 李容柏 

机构地区：[1]广西理工科学实验中心广西大学农学院,南宁530005 [2]亚热带农业生物资源保护与利用国家重点实验室,南宁530005 [3]湖南农业大学农学院,长沙410128

出　　处：《基因组学与应用生物学》2015年第6期1283-1289,共7页Genomics and Applied Biology

基　　金：广西自然科学基金项目(2013GXNSFBA019059);广西理工科学实验中心运行课题(YXKT2014015)共同资助

摘　　要：为筛选出在苗期和孕穗期都具有强耐冷性的种质以供水稻耐冷研究与育种利用,本研究利用人工低温胁迫的方法,对20份苗期耐冷的普通野生稻染色体片段代换系进行了孕穗期耐冷性鉴定评价。结果表明:(1)在20份代换系材料中,孕穗期耐冷性表现强(3级)、中(5级)和弱(7级)级别的材料为2份、15份和3份,分别占供试材料的10%、75%和15%;(2)水稻苗期和孕穗期的耐冷性呈显著正相关(p<0.05),所鉴定出的2份抗性3级材料(DC907和DC866)不但可以作为苗期耐冷性抗源,同时也可以作为孕穗期耐寒性抗源;(3)可溶性蛋白、可溶性糖、游离氨基酸、脯氨酸、丙二醛和超氧化物歧化酶等各项生理指标与相对结实率之间均无显著的相关关系,因此均不适宜作为孕穗期耐冷性的鉴定评价指标。To screen out the germplasm with strong cold tolerance both at seedling stage and booting stage, which was a base for the investigation and breeding of rice cold tolerance, 20 Oryza rufipogon-donated Chromosome Segment Substitution Lines(CSSLs) showing cold tolerance during seedling stage were analyzed and evaluated at booting stage under low temperature stress of artificial climate chamber. The results showed that:(1) A total of 2, 15 and 3 CSSLs showing strong, medium and poor on cold tolerance at booting stage were identified among20 CSSLs, which accounted for 10%, 75% and 15%, respectively;(2) The correlation analysis showed that a significant positive correlation(p<0.05) existed between the cold tolerances at seeding and booting stage. DC907 and DC866, viz., two CSSLs showing strong cold tolerance at booting stage, might be useful in rice improvement for cold tolerance at both seeding and booting stage;(3) The correlation analysis showed that soluble protein, soluble sugar, amino acid, proline, malondialdehyde(MDA), and superoxide dismutase(SOD) were not significantly correlated to relative seed setting rate, so they could not be selected to identify the cold tolerance at booting stage.

关 键 词：水稻 染色体片段代换系 孕穗期 耐冷性 评价 

分 类 号：S511.9[农业科学—作物学]