冬虫夏草寄主蒲氏钩蝠蛾β-1,3-葡聚糖识别蛋白家族的分子结构与功能分析  被引量:2

Molecular Structure and Function of β-1,3-Glucan Recognition Protein Family in Ophiocordyceps sinensis Host Thitarodes pui

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作  者:雷桅[1] 税晓容[2] 黄石安[1] 陈灿[1] 丁元林[3] 

机构地区:[1]广东医学院心血管疾病研究室,湛江524001 [2]广东医学院血管外科研究室,湛江524001 [3]广东医学院医学系统生物学研究所,东莞523808

出  处:《基因组学与应用生物学》2015年第6期1300-1305,共6页Genomics and Applied Biology

基  金:国家自然科学基金(81300035,81403044);广东省自然科学基金(S2013040012115)共同资助

摘  要:β-1,3-葡聚糖识别蛋白(βGRP)是昆虫响应真菌侵染的重要识别介质,能够识别并结合真菌细胞壁的β-1,3-葡聚糖从而激活体内的天然免疫系统。本文采用Protparam等生物信息学方法和工具对βGRP家族TpβGRP4a和TpβGRP4b基因及其编码蛋白的序列特征、分子结构、理化性质和生化功能等进行了预测分析。结果显示该基因的核苷酸序列全长分别为1 991 bp和1 681 bp,包括3'-UTR、ORF和5'-UTR序列,其蛋白分子式C2504H3832N672O736S15和C2094H3188N554O610S12,含496个和412个氨基酸残基,均以Gly含量最高,定位于分泌途径,无跨膜结构域,二级结构和三维模型解析完成,以随机卷曲为主,是含有21个碱基的信号肽和Glyco-hydro及Laminarinase功能域的亲水性蛋白质,而它们在稳定性、酸碱性和磷酸化位点存在不同,表明TpβGRP家族与目前已知βGR在昆虫对病原物的模式识别和免疫反应机制中的作用类似,但作用方式可能不同。本文为深入研究冬虫夏草寄主钩蝠蛾昆虫βGRP的生化特性和分子免疫学机理奠定基础,从而推动冬虫夏草资源的开发和保护,对丰富昆虫天然免疫体系也有积极意义。β-1,3-glucan recognition protein(βGRP) is an important recogition meditor by which insects response fungal infection, and it can recognize and bind to β-1,3-glucan and thus activates the innate immune system in vivo. In this study, the sequence characteristics, molecular structure, physicochemical properties and biochemical function of βGRP gene family, such as TpβGRP4a and TpβGRP4b, and their coding protein were predicted and analyzed by bioinformatic methods and tools like Protparam. The results showed that the length of nucleotide acid sequences were 1 991 bp and 1 681 bp, respectively including 3'-UTR, ORF and 5'-UTR; the molecular formula of the βGRP proteins were C2504H3832N672O736S15 and C2094H3188N554O610S12containing 496 and 412 aa, and Gly was the most abundant amino acid; βGRPs were anchored in the secretory pathway, without trans-membrane domain, and random coil is the main component after completion of secondary structures and three-dimensional modeling;furthermore, their were hydrophilic proteins with a 21 aa plastidial transit-peptide and the Glyco-hydro and Laminarinase domains. However, their stability, p H and phosphorylation sites are different each other. Therefore,βGRP family was similar to effects of the currently known βGRP in pattern recognition and immune response mechanism. This paper lays a foundation for study on the biochemical and molecular immunologic mechanism of Ophiocordyceps sinensis and its host insects Thitarodes, which contributes to promote the development and protection of O. sinensis, and also has the positive significance to enrich the natural immune system of insects.

关 键 词:冬虫夏草 蒲氏钩蝠蛾 β-1 3-葡聚糖识别蛋白 生物信息学 免疫 

分 类 号:S567.35[农业科学—中草药栽培] Q966[农业科学—作物学]

 

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