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作 者:刘盛楠[1] 邵淑丽[1] 张伟伟[1] 于秋芬[1] 苗长久[1] 奥列格 肖越[1] 李妍[1]
机构地区:[1]齐齐哈尔大学生命科学与农林学院,齐齐哈尔161006
出 处:《基因组学与应用生物学》2015年第8期1617-1624,共8页Genomics and Applied Biology
基 金:黑龙江省教育厅科学技术项目(批准号:12511611);黑龙江省自然科学基金(批准号:C201241)共同资助
摘 要:为了探讨紫檀芪(PTE)对人肝癌细胞增殖和凋亡的影响及其作用机制,肝癌细胞系Hep G2被选择作为研究对象,并对紫檀芪与其的作用进行了不同角度的研究。MTT法被用来检测不同浓度紫檀芪对Hep G2细胞活力的影响;倒置显微镜和流式细胞术检测紫檀芪处理后Hep G2细胞的凋亡情况;实时定量PCR和Western blot分别检测紫檀芪处理后Bax、Bcl-2、Fas、Cycs(细胞色素C)和Caspase3基因m RNA和蛋白表达情况。结果显示紫檀芪对Hep G2细胞增殖有很强的抑制作用,在最佳条件下,抑制率为(40.85±2.55)%。形态学观察发现紫檀芪作用后的细胞呈明显凋亡状态,凋亡率为(15.61±0.71)%,出现细胞周期阻滞现象。进一步的研究表明,经紫檀芪处理后,Hep G2细胞的线粒体膜电位明显下降(p<0.01),Bcl-2的表达增强,Bax、Fas、Cycs和Caspase3的表达明显降低(p<0.05)。这些实验事实暗示紫檀芪能够抑制Hep G2细胞增殖并促进其发生凋亡,其机制可能是通过上调Bax、Fas、Cycs和Caspase3基因,下调Bcl-2基因来实现的。To investigate the effects and mechanism of pterostilbene(PTE) on the proliferation and apoptosis of human hepatoma Hep G2 cell line, human liver carcinoma line Hep G2 cells were selected and several methods were applied. MTT assay was used to measure Hep G2 cells activity. Inverted microscope and FACScan were used to observe apoptosis. The levels of Bax, Bcl-2, Fas, Cycs and Caspase3 were determined by Real Time RT-PCR and Western blot. The results showed that PTE could inhibit the proliferation of Hep G2 cells on dose and time-depend manners, and the inhibition rate could be up to(40.85±2.55)%. The Hep G2 cells displayed typical morphological changes and induced arrest of the cell cycle and apoptosis after the treatments. The potential of ΔΨm declined, and the expressions of Bax, Fas, Cycs and Caspase3 were incraesed, however, the expression of Bcl-2 was decreased. The results implied that the PTE could inhibit the growth of Hep G2 cells and enhance cell apoptosis probably via upregulating Bax, Fas, Cycs, Caspase3 expressions and downregulating Bcl-2.
关 键 词:紫檀芪 HEPG2 细胞凋亡 Bcl-2 BAX FAS Cycs CASPASE3
分 类 号:R273[医药卫生—中西医结合]
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