微囊化异种肝细胞移植治疗大鼠急性肝功能衰竭  

Treatment of acute hepatic failure by transplantation of microencapsulated xenogenic hepatocyte

作　　者：张伟杰[1] 宫念樵[1] 李锦文[1] 马绪娴[1] 左利群[1] 郭晖[1] 李岗山[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院器官移植研究所,湖北省武汉市430030

出　　处：《世界华人消化杂志》2002年第12期1396-1398,共3页World Chinese Journal of Digestology

基　　金：国家教委教外司留;No.19990747;同济医院科研基金;No.2000017~~

摘　　要：目的:探讨微囊化猪肝细胞异种移植治疗大鼠急性肝功能衰竭的可行性.方法:将分离的猪肝细胞微囊化后移植到Wistar大鼠腹腔内,3d后切除大鼠80%肝叶造成肝衰模型,观察大鼠2wk存活情况.结果:对照组、未微囊化肝细胞移植组及微囊化肝细胞移植组的2wk存活率分别为6.2%,31.2%和56.2%,微囊化组肝细胞存活率明显较未微囊化组及对照组高.微囊化肝细胞在移植14d后结构基本完好.结论:微囊化异种肝细胞移植提供了一种治疗急性肝功能衰竭的可行方法.AIM:To evaluate the treatment effect of transplantation ofmicroencapsulated xenogenic hepatocyte on acute hepaticfailure.METHODS:The isolated porcine hepatocytes were encapsulatedin barium alginate microcapsule and intraperitoneally transplantedinto Wistar rats. 3 days later, 80 % of liver lobes wereresected. The survival rate of two weeks was comparedwith those of control (nontransplantation) and unencapsu-lated groups.RESULTS:The survival rate of two weeks in the group ofcontrol, unencapsulatation and microencapsulation were 6.25 %,31.2 % and 56.2 %, respectively. There were a significantdifference in survival rate between the encapsulated groupand other groups. The histologic structure of microencapsulatedporcine hepatocytes was basically normal 14 days followingtransplantation.CONCLUSION:Transplantation of microencapsulatedxenogenic hepatocyte provides a possibility in treatment ofacute hepatic failure.

关 键 词：微囊化异种肝细胞移植 治疗 大鼠 急性肝功能衰竭 

分 类 号：R657.3[医药卫生—外科学]