mtlD/gutD双价基因转化美洲黑杨×青杨的研究  

STUDIES ON TRANSFORMATION OF MTLD/GUTD DIVALENT GENES TO POPULUS DELTOIDS× P. CATHAYANA

作　　者：樊军锋[1] 韩一凡[2] 李玲[2] 彭学贤[3] 李嘉瑞[4] 

机构地区：[1]西北农林科技大学林学院,杨凌712100 [2]中国林业科学研究院林业研究所,北京100091 [3]中国科学院微生物研究所,北京100080 [4]西北农林科技大学园艺学院,杨凌712100

出　　处：《林业科学》2002年第6期30-35,共6页Scientia Silvae Sinicae

基　　金：国家林业局黄土高原林木培育实验室提供了部分资助

摘　　要：利用叶盘法开展了mtlD gutD双价基因转化美洲黑杨×青杨的研究 ,经诱导不定芽及诱导生根阶段卡那霉素 (选择性抗生素 )连续筛选 ,获得了 38株卡那霉素抗性植株。抗性植株经PCR检测 ,2 8株呈阳性。对其中 5株PCR阳性植株进行Southern及Western杂交 ,有 4株二者均呈阳性 ,证明双价基因成功整合到美洲黑杨×青杨基因组中并得到表达。耐盐实验表明这Transformation of mtlD/gutD divalent genes to Populus deltoids × P. cathayana by means of leaf disc was undertaken. Through successive selection in shoot and root induction stage under high level kanamycin pressure, 38 kanamycin\|resistant rooted plants were obtained. PCR analysis showed that 28 of these kanamycin\|resistant rooted plants were positive. 5 of these 28 PCR positive plants were taken out for Southern blot and Western blot, and the result indicated that 4 of the 5 plants were positive for both. This meant that mtlD/gutD divalent genes were successfully integrated into the genome of these 4 plants, and the proteins expressed by mtlD/gutD divalent genes were synthesized. The salt resistant test demonstrated that salt tolerance ability of these 4 plants was enhanced to some extent.

关 键 词：美洲黑杨×青杨 mtlD/gutD双价基因 基因转化 分子检测 耐盐性测定 卡那毒素 耐盐植物 盐域地开发利用 

分 类 号：S156.4[农业科学—土壤学] S332.[农业科学—农业基础科学]