4种刺激剂对小鼠腹腔巨噬细胞生成白细胞介素6的研究  

PRODUCTION AND SECRETION OF INTERLEUKIN 6 FROMSTIMULATED PERITONEAL MACROPHAGES OF THE MOUSE

作　　者：钟淼[1,2] 程桂芳[1,2] 王文杰[1,2] 陈洁[1,2] 朱秀媛 张均田[1,2] 

机构地区：[1]中国医学科学院 [2]中国协和医科大学药物研究所

出　　处：《药学学报》1997年第12期893-897,共5页Acta Pharmaceutica Sinica

摘　　要：用白细胞介素６（ｉｎｔｅｒｌｅｕｋｉｎ６，ＩＬ６）依赖细胞株Ｂ９的ＭＴＴ法，研究了４种刺激剂ＰＭＡ，Ａ２３１８７，ｆＭＬＰ和ＬＰＳ对小鼠腹腔巨噬细胞生成及分泌ＩＬ６的影响。分别检测了巨噬细胞培养上清液及胞溶部分ＩＬ６的含量。结果表明，ＰＭＡ（０１～１０μｍｏｌ·Ｌ－１），Ａ２３１８７（００１～１μｍｏｌ·Ｌ－１），ｆＭＬＰ（００２５～２５μｍｏｌ·Ｌ－１）均能促进巨噬细胞生成及分泌ＩＬ６，且有量效关系。ＬＰＳ（０１～１０μｇ·ｍｌ－１）能增加巨噬细胞培养上清液中ＩＬ６的含量，但对胞溶部分无影响。结果提示：巨噬细胞ＩＬ６的生成及分泌与蛋白激酶Ｃ（ＰＫＣ）和钙离子通道活化有关；In this paper, a bioassay method involving the interleukin 6 dependent murine hybridoma B9 cell line was used to detect the level of released and cellassociated interleukin 6 in resident peritoneal macrophages from the mouse. The phorbol ester, phorbol 12myristate 13acetate(PMA), the calcium ionophores A23187, the chemotactic peptide fMLP and lipopolysaccharide(LPS) were introduced in these experiments as stimulators. PMA, A23187 and fMLP were shown to induce a dosedependent increase of both released and cellassociated interleukin 6, whereas, LPS caused increase of interleukin 6 activity in supernatants of cultured peritoneal macrophages of the mouse. The effects of PMA and A23187 suggested that the pathway of PKC and calcium is involved in the production and secretion of interleukin 6. The effects of fMLP and LPS provided evidence that they participated in the process of inflammation. The difference between LPS and the other three stimulators in cellassociated interleukin 6 activity might suggest a different mechanism of actions of generation of interleukin 6.

关 键 词：白细胞介素6 B9细胞株 佛波酯 钙离子载体A23187 甲酰三肽 脂多糖 

分 类 号：R392.1[医药卫生—免疫学]