超临界流体萃取法毛细管气相色谱法分析牡丹皮及制剂中丹皮酚的含量  

ANALYSIS OF PAEONOL IN PAEONIA SUFFRUTICOSA ANDR. AND ITS PATENT PREPARATIONS BY SUPERCRITICAL FLUID EXTRACTION AND CAPILLARY GAS CHROMATOGRAPHY

作　　者：缪海均[1] 柳正良 李云华 

机构地区：[1]第二军医大学长征医院药材科

出　　处：《药学学报》1997年第12期928-930,共3页Acta Pharmaceutica Sinica

摘　　要：采用超临界流体萃取法（ｓｕｐｅｒｃｒｉｔｉｃａｌｆｌｕｉｄｅｘｔｒａｃｔｉｏｎ，ＳＦＥ）提取中药牡丹皮及其成方制剂中丹皮酚，以氯仿作改性剂，在温度９０℃，压力２８ＭＰａ下，二氧化碳动态萃取体积３ｍｌ；静态萃取时间５ｍｉｎ。此法简便快速，萃取完全。用大孔径毛细管柱气相色谱法作含量监测，结果：相关性好（γ＝０９９９９），中药与制剂的回收率分别为９７８％，ＲＳＤ＝２３５％（ｎ＝３）；１００３％，ＲＳＤ＝１８９％（ｎ＝３）。A supercritical fluid extraction (SFE) method was developed for the extraction of paeonol in Paeonia suffruticosa Andr. and its patent preparations. A systematic method was used to optimize five variables (temperature, pressure, modifier concentration, static extracting time and CO2 dynamic extracting volume) of SFE. Sample extracts were analyzed by widebore capillary gas chromatography (CGC). The method showed good linearity (γ=09999)in the range of 01～16 mg·ml-1 of paeonol. The average recovery was 978% with RSD=235% (n=3) for Paeonia suffruticosa andr., 1003% with RSD=189% (n=3) for its patent preparations.

关 键 词：超临界流体萃取法 牡丹皮 丹皮酚 毛细管柱气相色谱法 

分 类 号：R284.1[医药卫生—中药学]