山西汉族D17S30基因座的多态性研究  

Polymorphism of D17S30 locus of Han nationality population in Shanxi

作　　者：梁景青[1] 赵芳芳 张杰 梁景峰 

机构地区：[1]山西医科大学法医系,太原030001 [2]山西省公安厅 [3]山西省孝义市公安局 [4]山西省太原市五一百货集团医务室

出　　处：《山西医科大学学报》2002年第6期493-494,共2页Journal of Shanxi Medical University

摘　　要：目的　研究山西地区汉族人群D17S30基因座多态性。方法　以PCR技术 ,聚丙烯酰胺凝胶垂直电泳和银染法对小卫星区域D17S30位点的扩增片段长度多态性进行了研究。结果和结论　对山西地区 10 2名无关个体进行了D17S30位点的等位基因频率调查 ,发现 10个等位基因 ,片段长度分布于 16 8～ 798bp之间。基因频率分布于 0 0 0 98～ 0 2 745之间 ,杂合度为 0 82。对 10个家系进行分析符合孟德尔遗传规律 ,并获得本地区相应的群体遗传学数据 ,在医学、法医学个人识别和亲子鉴定中可发挥重要作用。Objective The distribution of D17S30 locus in Shanxi Han population was studied. Methods Using PCR, polyacryl gel vertical electrophoresis and silver staining. Results and conclusion The results showed that 10 alleles were detected .The allele fragment length varied from 168 to 798 bp.The allele frequency was 0.009 8～0.274 5 and heterozygosity was 0.82. Pedigree analysis indicates that alleles of D17S30 locus obey the Mendel's laws .Population genetic data of the D17S30 locus in Shanxi are obtained. This can be applied to clinical medicine paternity test and personal identification.

关 键 词：山西 基因频率 染色体图 多态现象 遗传学 D17S30位点 汉族人 

分 类 号：Q987.1[生物学—遗传学]