基于网络药理学探讨速效救心丸联合复方丹参注射液治疗不稳定型心绞痛的机制  

The mechanism of quick-acting heart-saving pill combined with compound Danshen injection in the treatment of unstable angina pectoris based on network pharmacology

作　　者：李雯莉[1] 高冉冉[1] 王莹[2] 曹阳 Li Wenli;Gao Ranran;Wang Ying;Cao Yang(Emergency and Trauma Center,the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830011,China)

机构地区：[1]新疆医科大学第一附属医院急救·创伤中心,新疆乌鲁木齐830011 [2]新疆医科大学第一附属医院医务部医务管理科,新疆乌鲁木齐830011

出　　处：《中国急救医学》2025年第1期47-53,共7页Chinese Journal of Critical Care Medicine

基　　金：新疆维吾尔自治区自然科学基金资助项目(2024D01C173)。

摘　　要：目的采用网络药理学和分子对接技术探索速效救心丸联合复方丹参注射液治疗不稳定型心绞痛的分子调控机制。方法中药系统药理学数据库与分析平台(TCMSP)、中医药百科全书平台(ETCM)、中药分子机制研究的生物信息学分析工具(BATMA-TCM)、本草组鉴平台(HERB)数据库结合成分检测文献筛选速效救心丸联合复方丹参注射液的成分和靶点,同时GeneCards、DisGeNET和OMIM数据库获取不稳定型心绞痛的靶点。用于进行基因本体和京都基因与基因组百科全书富集分析。结果本研究筛选出川芎嗪、丹参酮ⅡA、丹酚酸B等33种主要活性成分,得到前列腺素内过氧化物合成酶2、白细胞介素6(IL-6)等32个速效救心丸联合复方丹参注射液治疗不稳定型心绞痛的靶点。分子对接结果证明,本研究获得的活性成分与其对应的不稳定型心绞痛靶点间均有较高的亲和力。结论速效救心丸联合复方丹参注射液通过川芎嗪、丹参酮ⅡA、丹酚酸B等活性成分靶向IL-6、白细胞介素1β(IL-1β)、肿瘤坏死因子(TNF)等关键靶点发挥治疗不稳定型心绞痛的功效。Objective To explore the molecular regulatory mechanism of quick-acting heart-saving pill combined with compound Danshen injection in the treatment of unstable angina pectoris by using network pharmacology and molecular docking techniques.Methods The Traditional Chinese Medicine Systematic Pharmacology Database and Analysis Platform(TCMSP),the Encyclopedia of Traditional Chinese Medicine(ETCM),a Bioinformatics Annotation Database for Molecular Mechanism of Traditional Chinese Medicine(BATMA-TCM)and HERB databases in combination with compositional assay literatures were used to screen the components and targets of quick-acting heart-saving pill combined with compound Danshen injection,while GeneCards,DisGeNET and OMIM databases were used to obtain the targets of unstable angina pectoris.The Weishengxin platform was used to perform gene ontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)enrichment analyses.Results In this study,33 major active ingredients including chuanxiongzine,tanshinoneⅡA and salvianolic acid B were screened to obtain 32 targets for unstable angina pectoris treated with the combination of quick-acting heart-saving pill and compound Danshen injection,including prostaglandin endoperoxide synthase 2 and interleukin 6.The molecular docking results demonstrated that there was a high affinity between the active ingredients obtained in this study and their corresponding targets of unstable angina pectoris.Conclusions The efficacy of quick-acting heart-saving pill combined with compound Danshen injection in the treatment of unstable angina pectoris was demonstrated by targeting the key targets of IL-6,IL-1βand TNF with the active ingredients,such as chuanxiongzine,tanshinoneⅡA and salvianolic acid B.

关 键 词：速效救心丸 复方丹参注射液 不稳定型心绞痛 网络药理学 

分 类 号：R285[医药卫生—中药学]