一体化生物加工CBP产纤维乙醇研究进展  

Research progress in the production of cellulosic ethanol via consolidated bioprocessing

作　　者：徐凯 崔金娜 刘占英 XU Kai;CUI Jinna;LIU Zhanying(Inner Mongolia Autonomous Region Fermentation Industry Energy Conservation and Emission Reduction Engineering Technology Research Center,Hohhot 010051,Inner Mongolia,China;Biological Fermentation Green Manufacturing Inner Mongolia Autonomous Region Engineering Research Center,Hohhot 010051,Inner Mongolia,China;School of Chemical Engineering,Inner Mongolia University of Technology,Hohhot 010051,Inner Mongolia,China)

机构地区：[1]内蒙古自治区发酵产业节能减排工程技术研究中心,内蒙古呼和浩特010051 [2]生物发酵绿色制造内蒙古自治区工程研究中心,内蒙古呼和浩特010051 [3]内蒙古工业大学化工学院,内蒙古呼和浩特010051

出　　处：《化工进展》2024年第12期6873-6882,共10页Chemical Industry and Engineering Progress

基　　金：内蒙古杰出青年基金(2022JQ10);内蒙古自治区“草原英才”工程产业创新人才团队项目。

摘　　要：全球能源危机的升级使得开发可再生替代燃料变得更加迫切。一体化生物加工(consolidated bioprocessing,CBP)是一项整合了预处理、纤维素酶生产、纤维素水解糖化、己糖和戊糖发酵产醇四个单元操作为一体的生物加工技术,是降解木质纤维素生物质产纤维素乙醇最具前景的高效低成本策略。本文以细菌和真菌在CBP产纤维素乙醇中的研究为出发点,回顾了纤维素乙醇工业化的发展历程。综述了一体化生物加工技术中四种不同的策略,包括单菌CBP系统、自然微生物菌群、组合微生物群落、菌种代谢工程改造,系统总结了目前国内外报道的CBP底盘细菌与真菌的种类、发酵代谢特征及工程化和共培养策略,并论述了研究案例和发展现状。为新型功能菌株的选育和非粮生物燃料的发展提供理论依据。The escalation of the global energy crisis has made the development of renewable alternative fuels even more urgent.Consolidated bioprocessing(CBP)is a bioprocessing technology that integrates pre-treatment,cellulase production,cellulose hydrolysis and saccharification,and hexose and pentose fermentation to produce ethanol.It is the most promising and efficient low-cost strategy for degrading lignocellulosic biomass to produce cellulose ethanol.Starting from the research on bacteria and fungi in the production of cellulose ethanol from CBP,this article reviews the development process of cellulose ethanol industrialization.This article expounds four different strategies in the consolidated bioprocessing process,including single bacterial CBP system,natural microbial community,composite microbial community,and microbial metabolic engineering transformation.It systematically summarizes the types,fermentation metabolic characteristics,and engineering and co-cultivation strategies of CBP chassis bacteria and fungi reported in domestic and foreign literature,and discusses their research cases and development status.This article provides theoretical basis for the breeding of new functional strains and the development of non-grain biofuels.

关 键 词：一体化生物加工 纤维素乙醇 微生物菌群 生物燃料 生物质 合成生物学 

分 类 号：TK6[动力工程及工程热物理—生物能] Q81[生物学—生物工程] X71[环境科学与工程—环境工程] TQ923[轻工技术与工程—发酵工程]