耦合均相-异相类芬顿体系强化水体耐药伤寒沙门氏菌的杀灭  

Coupled homogeneous/heterogeneous Fenton-like system for enhanced inactivating of tetracycline-resistance Salmonella typhi

作　　者：曾湘楚 宁海潮 武哲 韦瑞松 银秀菊 ZENG Xiangchu;NING Haichao;WU Zhe;WEI Ruisong;YIN Xiuju(Guangxi Collaborative Innovation Center of Modern Sericulture and Silk,School of Chemistry and Bioengineering,Hechi University,Hechi 546300,Guangxi,China;Guangxi Key Laboratory of Sericulture Ecology and Applied Intelligent Technology,School of Chemistry and Bioengineering,Hechi University,Hechi 546300,Guangxi,China;Guangxi Colleges Universities Key Laboratory of Exploitation and Utilization of Microbial and Botanical Resources,School of Chemistry and Bioengineering,Hechi University,Hechi 546300,Guangxi,China;Lishui Institute of Agriculture and Forestry Sciences Chemical Biology Center,Lishui 323000,Zhejiang,China)

机构地区：[1]广西现代蚕桑丝绸协同创新中心,河池学院化学与生物工程学院,广西河池546300 [2]广西蚕桑生态学与智能化技术应用重点实验室,河池学院化学与生物工程学院,广西河池546300 [3]微生物及植物资源开发利用广西高校重点实验室,河池学院化学与生物工程学院,广西河池546300 [4]丽水市农林科学研究院化学生物中心,浙江丽水323000

出　　处：《化工进展》2024年第12期7078-7094,共17页Chemical Industry and Engineering Progress

基　　金：广西现代蚕桑丝绸协同创新中心基金(2023GXCSSC03);河池学院高层次人才科研启动项目(2019GCC008,2023GCC015,2021GCC031)。

摘　　要：由于抗生素排放衍生的抗生素耐药细菌(ARB)入侵问题是生态环境面临的一个新挑战。基于均相、异相体系活化过氧单硫酸盐(PMS)的类芬顿反应在水体四环素(TC)的降解中得到了广泛的研究,但是针对耐四环素细菌杀灭的研究甚少。本文发现水体共存Cu(Ⅱ)与TC形成络合物活化PMS的均相Cu(Ⅱ)/PMS体系能实现对耐四环素伤寒沙门氏菌(TRST)的有效杀灭,但杀灭率仅为52%左右。而在Cu(Ⅱ)-TC-TRST三元污染物中加入制备的N、S共掺杂磁性炭(N/S-MC)后,N/S-MC活化PMS的异相N/S-MC/PMS体系能实现均相Cu(Ⅱ)/PMS体系效果的叠加而大幅度促进对TRST的杀灭。尽管存在Cu(Ⅱ)和TC的竞争,耦合均相-异相类芬顿体系能强化复合污染水中对TRST的杀灭,杀灭率可提高至99%以上。并且其主要通过类芬顿反应产生的活性氧(ROS)破坏TRST的细胞膜、细胞壁及耐药基因(ARGs)而实现TRST的杀灭并阻断其耐药基因的水平转移。均相、异相类芬顿体系中自由基(·SO_(4)^(-)、·OH、·O_(2)^(-))和非自由基(^(1)O_(2))途径都参与了对TRST的杀灭,但呈现自由基的主导作用。本文提出了一种耦合均相-异相类芬顿体系,对水体耐药细菌的杀灭呈现良好的效果。The invasion of antibiotic resistance bacteria(ARB)from the discharge of antibiotics is an emerging challenge to ecological environment.Fenton-like reactions based on the activation of the degradation of tetracycline(TC)in water,however,there are few studies on the inactivating of tetracycline resistant bacteria.It was found that the homogeneous Cu(Ⅱ)/PMS system with coexisting Cu(Ⅱ)and TC to form cupric complex activated PMS could effectively inactivate tetracycline resistant Salmonella typhi(TRST),but the inactivation efficiency was only about 52%.After adding N/S co-doped magnetic carbon(N/S-MC)to Cu(Ⅱ)-TC-TRST ternary contaminants,the heterogeneous N/S-MC/PMS system could realize the superposition on the effect of homogeneous Cu(Ⅱ)/PMS system and greatly promote the inactivation of TRST.Despite the competition of Cu(Ⅱ)and TC,the coupling homogeneous and heterogeneous Fenton-like system could enhance the inactivation efficiency of TRST in the complex polluted water,and that increased to more than 99%.The ROS produced by Fenton-like reaction destroyed the cell membrane,cell wall and drug resistance genes(ARGs)of TRST,furthermore prevented its horizontal transfer.In the homogeneous and heterogeneous Fenton-like systems,both free radical(·SO_(4)^(-),·OH,·O_(2)^(-))and non-free radical(1O_(2))pathways participated in the inactivating of TRST,but exhibited the dominant role of free radicals.In this paper,a coupled homogeneous and heterogeneous Fenton-like system was proposed,which showed good efficacy in inactivating ARB in water.

关 键 词：类芬顿反应 过氧单硫酸盐 均相和异相 耐药细菌 炭基催化剂 

分 类 号：X522[环境科学与工程—环境工程]