解毒化瘀颗粒对肝衰竭大鼠的保护作用及机制研究  

Study on Protective Effect and Mechanism of Jiedu Huayu Granules(解毒化瘀颗粒)on Liver Failure in Rats

作　　者：柳琳琳[1] 杨玉雯 姚元谦 毛德文[2] 张文富[2] 潘哲[2] 吕建林[2] LIU Linlin;YANG Yuwen;YAO Yuanqian;MAO Dewen;ZHANG Wenfu;PAN Zhe;LYU Jianlin(Guangxi University of Chinese Medicine,Nanning 530001,Guangxi,China;The First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning 530023,Guangxi,China)

机构地区：[1]广西中医药大学,广西南宁530001 [2]广西中医药大学第一附属医院,广西南宁530023

出　　处：《中华中医药学刊》2025年第1期69-71,I0011,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81860839);广西自然科学基金项目(2020GXNSFAA238020,2022GXNSFAA035446);广西研究生教育创新计划项目(JGY2021110,YCXJ2021059,YCXJ2021065);广西高校中青年教师(科研)基础能力提升项目(2021KY0300);广西中医药大学引进博士科研启动基金项目(2023BS023)。

摘　　要：目的观察解毒化瘀颗粒对肝衰竭大鼠的保护作用,并探讨其作用机制。方法将30只SPF级Wistar大鼠按随机数字表法分为空白组、模型组、解毒化瘀颗粒组,每组10只。采用D-氨基半乳糖(D-GalN)+内毒素脂多糖(LPS)构建急性肝衰竭大鼠模型。解毒化瘀颗粒组于造模前5 d开始灌胃给药,空白组与模型组分别给予等量蒸馏水,延续至成模后24 h。检测各组大鼠肝功能[丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、总胆红素(TBil)],氧化应激指标[丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)],炎症因子[白介素6(IL-6)、肿瘤坏死因子α(TNF-α)]水平;HE染色观察各组大鼠肝组织病理变化。结果经解毒化瘀颗粒治疗后大鼠肝组织肝细胞坏死的情况得到明显改善,同时可明显降低模型大鼠ALT、AST、TBil、MDA、IL-6、TNF-α,提高SOD、GSH-PX水平(P<0.05)。结论解毒化瘀颗粒具有良好的抗氧化抗炎作用,能减轻肝衰竭中的肝细胞坏死。Objective To observe the protective effect of Jiedu Huayu Granules(解毒化瘀颗粒)on liver failure in rats and to investigate its mechanism of action.Methods Thirty SPF-grade Wistar rats were divided into blank control group,model control group and Jiedu Huayu Granules group(JDHYKL group)according to the number table method,with 10 rats in each group.D-galactosamine(D-GalN)+lipopolysaccharide(LPS)was used to replicate the rat model of acute liver failure,and the JDHYKL group was given Jiedu Huayu Granules by irrigated 5 days before modeling and continued until 24 h after modeling,and the blank group and the model control group were given equal amounts of distilled water.The liver function[alanine aminotransferase(ALT),aspartate aminotransferase(AST),total bilirubin(TBil)],the levels of oxidative stress indexes[malondialdehyde(MDA),superoxide dismutase(SOD),glutathione peroxidase(GSH-PX)],inflammatory cytokines[interleukin-6(IL-6)and tumor necrosis factorα(TNF-α)]were detected in each group,and the histopathological changes of liver were observed in each group by HE staining.Results The hepatocyte necrosis in the liver tissue of rats was significantly improved after treatment with Jiedu Huayu Granules.Meanwhile,it could significantly decrease the levels of ALT,AST,TBil,MDA,IL-6 and TNF-α,and increase the levels of SOD and GSH-PX(P<0.05).Conclusion Jiedu Huayu Granules have significant antioxidant and anti-inflammatory effects,and could mitigate hepatocyte necrosis in liver failure.

关 键 词：肝衰竭 解毒化瘀颗粒 抗氧化 抗炎 

分 类 号：R289.5[医药卫生—方剂学]