电针治疗对缺血性脑卒中模型大鼠肠道菌群及炎症免疫功能的影响研究  

Effects of Electroacupuncture on Intestinal Flora and Inflammatory Immune Function in Ischemic Stroke Model Rats

作　　者：张蕴 林丹丹 王钦敏 高诗晴 ZHANG Yun;LIN Dandan;WANG Qinmin;GAO Shiqing(School of Education,Fujian Normal University of Technology,Fuzhou 353000,Fujian,China;Outpatient Center of Treatment and Prevention,Fujian Academy of Traditional Chinese Medicine,Fuzhou 350000,Fujian,China;School of Medicine,Fujian Health Vocational and Technical College,Fuzhou 350101,Fujian,China;Fuzhou Children's Hospital of Fujian Province,Fuzhou 350001,Fujian,China)

机构地区：[1]福建技术师范学院教育学院,福建福州353000 [2]福建省中医药科学院门诊部,福建福州350000 [3]福建卫生职业技术学院医学院,福建福州350101 [4]福建省福州儿童医院,福建福州350001

出　　处：《中华中医药学刊》2025年第1期204-209,共6页Chinese Archives of Traditional Chinese Medicine

基　　金：福建省自然科学基金青创项目(2022J05201);福建省中青年教师教育科研项目(JAT191300)。

摘　　要：目的探究电针治疗对缺血性脑卒中模型大鼠肠道菌群及炎症免疫功能的影响。方法按随机数字表法将16只Sprague Dawley(SD)大鼠分成假手术组、大脑中动脉闭塞(middle cerebral artery occlusion,MCAO)组、MCAO+电针组、MCAO+电针非穴组。采用线栓法进行左侧MCAO手术,制备脑缺血再灌注动物模型,手术完成后将其放入木屑中并温水保温观察其变化,2 h后将线栓缓慢退出,假手术组仅将动脉分离,然后缝合。造模24 h后,MCAO+电针组,选取神庭、百会穴进行针刺,应用SDZ-V电针仪,采用疏密波,30 min/次,1次/d,一共干预7 d;假手术组和MCAO组不予任何治疗,给予同等条件的喂养和抓取;MCAO+电针非穴组取胁下非经非穴点,在造模后24 h进行电针干预14 d。观察造模后24 h及3、7、14 d各组大鼠神经功能情况,检测干预14 d后大鼠脑含水量变化;造模成功后,采用longa法进行神经功能缺损评分;Morris水迷宫实验评价大鼠空间学习、记忆能力;Western blot检测各组大鼠缺血半暗带神经元生长相关蛋白;实时荧光定量聚合酶链式反应(polymerasec chain reaction,PCR)测定粪便肠道菌群变化;双抗体夹心(ABC-enzyme-linked immunosorbent assay,ABC-ELISA)法检测血清和脑组织中的白细胞介素-1β(interleukin-1β,IL-1β)和细胞间黏附分子-1(intercellular adhesion molecule-1,ICAM-1)的含量,并分析IL-1β、ICAM-1与肠道菌群的相关性。结果在造模后24 h及3、7、14 d评估各组大鼠的神经功能情况,电针各组大鼠脑缺血再灌注后同期神经功能缺损评分均低于同期MCAO组,其中电针穴位组均明显低于同期非穴组(P<0.01),而电针组大鼠神经功能随着干预时间延长改善更明显。与MCAO组比较,MCAO+电针穴位组同期逃避潜伏期明显缩短(P<0.05),MCAO+电针穴位组穿越平台次数升高(P<0.05)。与MCAO组比较,电针组、电针非穴组大鼠生长相关蛋白-43(growth associated protein-43,GAP-43)、神经丝蛋白-200Objective To explore the effects of electroacupuncture therapy on intestinal flora and inflammatory immune function in ischemic stroke model rats.Methods According to random number table method,16 Sprague Dawley(SD)rats were divided into sham operation group,middle cerebral artery occlusion(MCAO)group,MCAO+electroacupuncture group and MCAO+electroacupuncture non-acupoint group.Left MCAO operation was conducted with wire occlusion method to prepare brain ischemia-reperfusion animal model.After the operation,the animal model was put into wood shavings and kept warm water to observe the changes.After 2 hours,the wire occlusion was slowly withdrawn,while only the arteries were separated and then sewed up in the sham operation group.24 hours after modeling,the MCAO+electroacupuncture group selected Shenting(DU24)and Baihui(DU20)and applied SDZ-V electroacupuncture instrument with density wave(1~20 Hz)once a day,for a total of 7 days.The sham operation group and the MCAO group were given the same feeding and grasping conditions without any treatment.In the MCAO+electroacupuncture non-acupoint group,the hypocholic non-meridian and non-acupoint points were selected,and the electroacupuncture intervention was carried out 24 hours after modeling for 14 days.The neural function of rats in each group was observed 24 h and 3,7 and 14 days after the modeling,and the change of brain water content was detected after the intervention for 14 days.After successful modeling,longa method was used to score the neurological impairment.Morris water maze experiment evaluated the spatial learning and memory ability of rats.Western blot analysis was performed to detect the growth related proteins of ischemic penumbra neurons in each group.Real-time fluorescence quantitative Polymerase Chain Reaction(PCR)was used to detect the changes of intestinal flora in rat stool.The contents of interleukin-1β(IL-1β)and intercellular adhesion molecule-1(ICAM-1)in serum and brain tissue were detected by double antibody sandwich ABC-enzyme-linked immuno

关 键 词：电针 缺血性脑卒中 肠道菌群 炎症免疫 

分 类 号：R245.97[医药卫生—针灸推拿学]