基于高效液相色谱法检测茜草炮制前后异茜草素的含量及意义  

Content and Significance of Xanthopurpurin in Rubia cordifolia before and after Processing Based on HPLC

作　　者：王娟弟 张明童 李欣 刘蕊 郭晓霞 李冬华 马潇 朱瑞娟 郭朝晖 马双成[4] 魏峰[4] 程显隆[4] WANG Juandi;ZHANG Mingtong;LI Xin;LIU Rui;GUO Xiaoxia;LI Donghua;MA Xiao;ZHU Ruijuan;GUO Zhaohui;MA Shuangcheng;WEI Feng;CHENG Xianlong(Gansu Institute for Drug Control,Lanzhou 730070,China;NMPA Key Laboratory for Quality Control of TCM,Lanzhou 730070,China;Shandong Vocational Animal Science and Veterinary College,Weifang 261061,China;National Institutes for Food and Drug Control,Beijing 100050,China)

机构地区：[1]甘肃省药品检验研究院,兰州730070 [2]国家药品监督管理局中药材及饮片质量控制重点实验室,兰州730070 [3]山东畜牧兽医职业学院,潍坊261061 [4]中国食品药品检定研究院,北京100050

出　　处：《世界中医药》2024年第19期2884-2888,共5页World Chinese Medicine

基　　金：国家重点研发计划“中医药现代化”重点专项(2023YFC3504105);国家中药材及饮片质量控制重点实验室项目(2023GSMPA-KL06)。

摘　　要：目的:建立高效液相色谱法,对比茜草炒制前后异茜草素含量变化,探讨其意义。方法:采用The ACE Excel 5 C18-PFP为色谱柱;以乙腈-水(60∶40)为流动相;流速1.0 mL/min;检测波长为245 nm;柱温30℃。委托榆林广济堂医药科技有限责任公司、安国市聚药堂药业有限公司、兰州安泰堂中药饮片有限公司、河北仁心药业有限公司、桐君堂药业有限公司对不同产地茜草进行炮制。检测茜草炒制前后异茜草素含量。结果:茜草生品中异茜草素的含量为0.040~0.169 mg/g;茜草炭(T=110℃~130℃)中异茜草素含量为0.309~0.380 mg/g;茜草炭(T=350~370℃)中的异茜草素含量为0.513~1.169 mg/g。异茜草素在6.712~83.905μg/mL间呈良好的线性,r为1.0000。平均回收率为101.3%,相对标准偏差(RSD)为2.1%。结论:高效液相色谱法可通过异茜草素含量有效判别茜草生品与茜草炭。Objective:To establish a high-performance liquid chromatography(HPLC)method for comparing the changes in the content of xanthopurpurin before and after the processing of Rubia cordifolia and to explore its significance.Methods:The analysis was performed using an ACE Excel 5 C18-PFP column with a mobile phase of acetonitrile-water(60:40)at a flow rate of 1.0 mL/min.The detection wavelength was set at 245 nm,and the column temperature was maintained at 30℃.The processing of R.cordifolia from different regions was entrusted to Yulin Guangjitang Pharmaceutical Technology Co.,Ltd.,Anguo Juyaotang Pharmaceutical Co.,Ltd.,Lanzhou Antaitang Traditional Chinese Medicine Decoction Pieces Co.,Ltd.,Hebei Renxin Pharmaceutical Co.,Ltd.,and Tongjuntang Pharmaceutical Co.,Ltd.The content of xanthopurpurin before and after processing was determined.Results:The content of xanthopurpurin in raw R.cordifolia ranged from 0.040 to 0.169 mg/g.After processing into charred R.cordifolia at 110℃to 130℃,the content ranged from 0.309 to 0.380 mg/g,and at 350℃to 370℃,the content ranged from 0.513 to 1.169 mg/g.Xanthopurpurin showed good linearity in the range of 6.712 to 83.905μg/mL,with a correlation coefficient(r)of 1.0000.The average recovery rate was 101.3%,with a relative standard deviation(RSD)of 2.1%.Conclusion:The HPLC method can effectively distinguish between raw R.cordifolia and its charred product based on the content of xanthopurpurin.

关 键 词：高效液相色谱法 异茜草素 茜草 茜草生品 茜草炭 炮制品 产地 质量评价 

分 类 号：R283[医药卫生—中药学]