商洛香菇和羊肚菌多糖提取工艺探究  

Study on Extraction Process of Polysaccharides from Shangluo Mushroom and Morchella esculenta

作　　者：王凤娟[1] 秦绍龙 WANG Feng-juan;QIN Shao-long(Shangluo University,Shangluo,Shaanxi 726000;Health Product Research Innovation Team,Shangluo,Shaanxi 726000)

机构地区：[1]商洛学院,陕西商洛726000 [2]商洛保健食品开发研究创新团队,陕西商洛726000

出　　处：《安徽农业科学》2025年第1期158-162,共5页Journal of Anhui Agricultural Sciences

基　　金：商洛学院保健食品开发研究创新团队项目(18SCX005);陕西省科技计划项目“商洛立体农业系统优化设计与调控增益技术研究”(2014KJXX-79)。

摘　　要：[目的]探索商洛香菇和羊肚菌中多糖的提取工艺。[方法]以商南县香菇和羊肚菌为原料,采用超声波辅助热水浸提法对多糖进行提取。通过单因素试验,对比料液比、超声时间、超声温度和热水浸提温度对香菇和羊肚菌多糖提取率的影响,借助正交试验,得出超声波辅助热水浸提法提取香菇和羊肚菌多糖的最佳提取工艺。[结果]在料液比1∶60,超声时间30 min,超声温度40℃,浸提温度60℃的条件下,香菇多糖提取率最高;在料液比1∶50,超声时间20 min,超声温度40℃,浸提温度50℃的条件下,羊肚菌多糖提取率最高。[结论]超声波辅助热水浸提法提取香菇和羊肚菌多糖是一种可行有效、节约、经济的提取方法。[Objective]In order to explore the extraction technology of polysaccharide from Lentinula edodes and Morchella esculenta in Shangluo.[Method]Polysaccharides were extracted from Lentinula edodes and Morchella esculenta in Shangnan County by ultrasonic assisted hot water extraction.Through single factor experiment,the effects of solid-liquid ratio,ultrasonic time,ultrasonic temperature and hot water extraction temperature on the extraction rate of Lentinula edodes and Morchella esculenta were compared.The orthogonal experiment was designed to obtain the optimum extraction process of Lentinula edodes and Morchella esculenta by ultrasonic-assisted hot water extraction.[Result]The results showed that the extraction rate of lentinan was the highest under the conditions of solid-liquid ratio 1∶60,ultrasonic time 30 min,ultrasonic temperature 40℃and extraction temperature 60℃.Under the conditions of solid-liquid ratio of 1∶50,ultrasonic time of 20 min,ultrasonic temperature of 40℃and extraction temperature of 50℃,the extraction rate of polysaccharide from M.esculenta was the highest.[Conclusion]Ultrasonic assisted hot water extraction of Lentinula edodes and Morchella esculenta polysaccharide is a feasible,effective,economical extraction method.

关 键 词：超声波辅助提取 提取工艺 多糖 正交试验 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]