大豆TrxG基因家族鉴定及表达模式分析  

Identification and expression pattern analysis of soybean TrxG gene family

作　　者：姜玲 阳小凤[1] 彭明[1] 顾小燕 周虹[1] JIANG Ling;YANG Xiaofeng;PENG Ming;GU Xiaoyan;ZHOU Hong(Crop Research Institute of Hunan Province,Changsha 410125,China)

机构地区：[1]湖南省作物研究所,长沙410125

出　　处：《广西植物》2024年第12期2222-2231,共10页Guihaia

基　　金：湖南省自然科学基金(2022JJ40258);湖南省农业科技创新资金项目(2023CX05);湖南省重点研发项目(2022NK2001)。

摘　　要：为探究TrxG(trithorax group)基因在大豆中的分布及响应大豆非生物胁迫的调控机制,该文通过生物信息学方法和实时荧光定量PCR技术对大豆GmTrxG基因家族成员的理化性质、系统进化关系、结构域组成、基因表达情况进行了分析。结果表明:(1)在大豆中共鉴定到15个GmTrxG基因家族成员,编码氨基酸长度为982~2394 aa的亲水性蛋白。(2)GmTrxG蛋白成员可被细分为3个亚族,并且每个亚族成员均含有1个SET结构域。(3)绝大多数GmTrxG基因在大豆茎尖、叶、花中的表达量较高。(4)GmSDG2a/b/c/d基因在高温、干旱胁迫下持续高表达;GmSDG2b/c和GmSDG14c受低温胁迫诱导表达;GmSDG2a/b、GmSDG14c和GmSDG25a/b受盐害胁迫表达上调。(5)大多数GmTrxG基因在茉莉酸(JA)和水杨酸(SA)激素处理下表达上调。由此可见,GmTrxG基因受到不同非生物胁迫的差异调控,成员GmSDG2a/b/c/d、GmSDG14c和GmSDG25a/b可能在大豆非生物胁迫响应中发挥重要作用。该研究结果为进一步探究大豆GmTrxG基因家族成员的生物学功能提供了科学依据。TrxG(trithorax group)gene family is conserved and widely existed in plants and animals,and plays important roles in regulating gene expression and maintaining normal growth and development of organisms.In order to investigate the distribution of TrxG genes in soybean and its regulatory mechanism in response to abiotic stress of soybean,the physicochemical properties,phylogenetic relationship,composition of domains and genes expression of soybean GmTrxG gene family were analyzed by bioinformatics method and real-time fluorescence quantitative PCR.The results were as follows:(1)A total of 15 GmTrxG gene family members were identified in soybean,encoding hydrophilic proteins with the length of 982-2394 aa amino acids.(2)GmTrxG protein members could be divided into three subfamilies,and members of each subfamily contained a SET domain.(3)The expression levels of most GmTrxG genes were highly expressed in stem tips,leaves and flowers of soybean.(4)The genes GmSDG2a/b/c/d were constantly and highly expressed by heat shock and drought.GmSDG2b/c and GmSDG14c were strongly induced by the stress of low temperature,and the genes GmSDG2a/b,GmSDG14c and GmSDG25a/b were up-regulated by salt.(5)Most GmTrxG genes were up-regulated under the treatment of jasmonic acid(JA)and salicylic acid(SA).In conclusion,GmTrxG genes are differentially regulated by different abiotic stresses,and members GmSDG2a/b/c/d,GmSDG14c and GmSDG25a/b may play an important role in soybean response to abiotic stress.The results provide a scientific reference for further exploring the biological function of soybean GmTrxG gene family members.

关 键 词：大豆 GmTrxG 基因家族 非生物胁迫 激素诱导 表达模式 

分 类 号：Q943[生物学—植物学]