应用rDNA序列分型实现胶红酵母分子流行病学本地化调查  

Localization investigation of molecular epidemiology of Rhodotorula mucilaginosa using rDNA sequence typing

作　　者：朱万骥 唐朝贵[2] 林宁[2] 郭建[3] 崔兰英[4] 肖玉玲[5] 徐慧[6] 严岩[7] 鲁怀伟 李宁 冀旭峰[10] 徐英春[11] 黄晶晶 ZHU Wanji;TANG Chaogui;LIN Ning;GUO Jian;CUI Lanying;XIAO Yuling;XU Hui;YAN Yan;LU Huaiwei;LI Ning;JI Xufeng;XU Yingchun;HUANG Jingjing(Department of Gastrointestinal Surgery,the Affiliated Huai'an No.1 People's Hospital of Nanjing Medical University,Huai'an 223300,China;Department of Clinical Laboratory,the Affiliated Huai’an No.1 People’s Hospital of Nanjing Medical University,Huai’an 223300,China;Department of Laboratory Medicine,South Division Shanghai East Hospital Affiliated to Tongji University,Shanghai 200120,China;Department of Laboratory Diagnosis,the First Affiliated Hospital of Harbin Medical University,Harbin 150081,China;Department of Laboratory Medicine,West China Hospital,Sichuan University,Chengdu 610041,China;Department of Clinical Laboratory,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China;Department of Clinical Laboratory,Peking University First Hospital,Beijing 100034,China;Department of Clinical Laboratory,The First Affiliated Hospital of USTC,University of Science and Technology of China(Anhui Provincial Hospital),Hefei 230001,China;Department of Laboratory Medicine,Provincial Hospital Affiliated to Fuzhou University,Fuzhou 350001,China;Department of Clinical Laboratory,The First Hospital of Jilin University,Changchun 130000,China;Department of Clinical Laboratory,Chinese Academy of Medical Sciences&Peking Union Medical College Hospital,Beijing 100730,China)

机构地区：[1]南京医科大学附属淮安第一医院胃肠外科,淮安223300 [2]南京医科大学附属淮安第一医院医学检验科,淮安223300 [3]上海市东方医院南院检验科,上海200120 [4]哈尔滨医科大学附属第一医院检验科,哈尔滨150081 [5]四川大学华西医院检验科,成都610041 [6]郑州大学第一附属医院检验科,郑州450052 [7]北京大学第一医院检验科,北京100034 [8]中国科学技术大学附属第一医院(安徽省立医院),合肥230001 [9]福州大学附属省立医院检验科,福州350001 [10]吉林大学第一医院检验科,长春130000 [11]中国医学科学院北京协和医院检验科,北京100730

出　　处：《中国真菌学杂志》2024年第6期541-547,共7页Chinese Journal of Mycology

基　　金：南京医科大学附属淮安第一医院科技创新团队(YCT 202302)。

摘　　要：目的为满足监测单位实时调查胶红酵母分子流行病学,筛选高效、快速、经济的分子分型方法,以实现胶红酵母临床分离株的分子流行病学本地化监测。方法收集中国医院侵袭性真菌病监测网(CHIF-NET)2010—2019年19所医院引发侵袭性感染的胶红酵母临床分离株73株,以及纳入21株环境分离株,基于DNA序列特征,筛选可本地化应用的胶红酵母分子流行病学监测方法。结果结合rDNA上ITS序列和D1/D2序列共鉴定出12个rDNA序列型,59.6%(56/94)的菌株为rDNA序列2型,其中流行簇菌株占比92.9%(52/56);73个临床分离株共鉴定出49个微卫星型别,分辨力为0.986,流行簇菌株分属30个微卫星型别。结论应用rDNA序列分型可实现胶红酵母流行簇菌株的本地化甄别。当监测到rDNA序列2型胶红酵母临床株时,提示流行株在医院环境中持续存在。

关 键 词：胶红酵母 分子流行病学 本地化 ITS D1/D2 

分 类 号：R379.9[医药卫生—病原生物学]