烟雾吸入性损伤复合减压病的动物模型建立  

Establishment of animal model of smoke inhalation injury combined with decompression sickness

作　　者：伍国胜 赖宏豪 陈锐勇 刘光盛 刘敏 何颖 Wu Guosheng;Lai Honghao;Chen Ruiyong;Liu Guangsheng;Liu Min;He Ying(Department of Burn Surgery,The First Affiliated Hospital of Naval Medical University,Shanghai 200433,China)

机构地区：[1]海军军医大学第一附属医院烧伤外科,上海200433 [2]海军特色医学中心

出　　处：《海军医学杂志》2024年第12期1261-1265,共5页Journal of Navy Medicine

基　　金：军队后勤科研计划重点项目(BHJ16J021);全军医学科技青年培育计划(21QNPY040)。

摘　　要：目的 探讨吸入性损伤复合减压病(DCS)的造模方法和评价指标,为开展吸入性损伤复合DCS损伤效应和作用机制、防治药物研究和卫勤处置奠定基础。方法 选取生理状态相似的雄性SD大鼠16只,随机分为空白对照组、DCS组、烟雾吸入性损伤组、复合伤组(采用自制装置建立烟雾吸入性损伤后复合DCS),于造模后24 h麻醉后解剖取材,行肺组织病理染色,并检测动脉血气、肺组织湿干比值、肺泡灌洗液炎症因子白细胞介素(IL)-1β、IL-6、肿瘤坏死因子α(TNF-α)的含量。结果 动脉血气分析显示复合伤组大鼠动脉血氧分压低于空白对照组、DCS组、烟雾吸入性损伤组,而二氧化碳分压高于上述3组,差异均有统计学意义(P<0.05)。肺组织病理学显示,复合伤组肺间隔增厚,炎症细胞大量聚集,肺泡内渗出,急性肺损伤征象较DCS组和烟雾吸入性损伤组更明显。复合伤组肺组织湿干比值、肺泡灌洗液炎症因子水平高于DCS组及烟雾吸入性损伤组(P<0.05)。结论 成功建立烟雾吸入性损伤复合DCS的动物模型,复合伤模型肺损伤程度重于单一损伤模型。Objective To explore animal model and evaluation indices of inhalation injury combined with decompression sickness(DSC),so as to lay a foundation for the studies on damage effect,mechanism,prevention,and military medical service of this disease.Methods A total of 16 SD rats with similar physiological states were selected and randomly divided into four groups,including control group,DCS group,smoke inhalation injury(SII)group,and combined injury group(DCS after SII induced by a self‐made device).Arterial blood gas,wet‐dry ratio of lung tissue(W/D),and inflammatory cytokines in bronchoalveolar lavage fluid(BALF)like interleukin(IL)‐1βIL‐6,and tumor necrosis factor‐α(TNF‐α)were measured at 24 hours after modeling.And pulmonary histopathological staining was performed.Results The partial pressure of oxygcn in the combined injury group was lower than those in the other groups,while the partial pressure of arterial oxygen in the combined injury group was higher than those in the other groups(P<0.05).The results of pulmonary histopathology showed the thickening of the interlobular septa and the leakage and massive accumulation of inflammatory cells.The symptoms of acute lung injury in the combined injury group were more severe than those in the DCS and SII groups.The W/D and inflammatory cytokines in the combined injury group were significantly higher than those in the DCS group and SII group(P<0.05).Conclusion The animal model of SII combined with DCS has been successfully established.Pulmonary injury in the combined injury model is more severe than that in the single injury models.

关 键 词：复合伤 吸入性损伤 减压病 动物模型 

分 类 号：R845.21[医药卫生—航空、航天与航海医学]