不同甘蔗品种组培苗光合自养生根特性  

Differences in Photosynthetic Autotrophic Rooting among Tissue Cultured Seedlings of Different Sugarcane Varieties

作　　者：刘丽敏 刘红坚 何为中 翁梦苓 高轶静 黄永才 LIU Limin;LIU Hongjian;HE Weizhong;WENG Mengling;GAO Yijing;HUANG Yongcai(Sugarcane Research Institute of Guangxi Academy of Sciences/Guangxi Key Laboratory of Sugarcane Genetic Improvement/Key Laboratory of Sugarcane Biotechnology and Genetic Improvement(Guangxi)of Ministry of Agriculture and Rural affairs,Nanning 530007,Guangxi,China;Guangxi Crop Genetic Improvement and Biotechnology Laboratory,Nanning 530007,Guangxi,China)

机构地区：[1]广西壮族自治区农业科学院甘蔗研究所/广西甘蔗遗传改良重点实验室/农业农村部广西甘蔗生物技术遗传改良重点实验室,广西南宁530007 [2]广西作物遗传改良重点实验室,广西南宁530007

出　　处：《甘蔗糖业》2024年第6期7-13,共7页Sugarcane and Canesugar

基　　金：国家糖料产业技术体系甘蔗宿根栽培岗位科学家项目(CARS-17);蔗糖产业省部共建协同创新中心(201812639);广西农业科学院基本科研业务专项(桂发科2021YT008,桂农科2020YM25)。

摘　　要：甘蔗组织培养技术已成为加速甘蔗优良品种繁育和推广应用的有效技术,组培苗光合自养生根技术可以减少生根步骤,缩短生根时间,降低生产成本,但是各个品种之间存在很大的差异,为了了解不同品种的生根特性,进一步探讨其生根差异的原因,本文以桂糖28号、桂糖29号、桂糖42号、桂糖44号、桂糖45号、新台糖22号无根组培苗为材料,喷施生根液后进行光合自养生根,通过调查组培苗的生根率、死苗率、存活率及生长状况指标,比较品种之间的生根差异。各试验数据的结果表明:参试6个品种之中,桂糖29号和桂糖44号生根早,抗逆性强,存活率达90%以上,属于易生根品种;桂糖45号、新台糖22号和桂糖42号生根时间稍迟,抗逆性中等,存活率在70%左右,属于中等易生根品种;桂糖28号生根迟,死苗率高,存活率仅有9.6%,为难生根品种。这些不同生根特性的品种为进一步研究光合自养生根机理提供材料。Sugarcane tissue culture technology has become an effective method for accelerating the breeding and widespread adoption of superior sugarcane varieties.Photosynthetic autotrophic rooting in tissue culture seedlings can reduce the steps required for rooting,shorten the rooting period,and lower production costs.However,significant variations exist among different varieties.In order to explore the rooting characteristics of different varieties and further screen the root for varieties that are difficult or easily,this study used rootless tissue culture seedlings of Guitang28,Guitang29,Guitang42,Guitang44,Guitang45,and ROC22 as materials.After applying a rooting solution,photosynthetic autotrophic rooting was carried out.By investigating indicators such as the rooting rate,seedling mortality rate,survival rate,and growth status,the rooting differences among varieties were compared.The results from various experimental data indicate that among the six tested varieties,Guitang29 and Guitang44 have early rooting initiation,strong stress resistance,and survival rates exceeding 90%,classifying them as easily rootable varieties;Guitang45、ROC22 and Guitang42 have a slightly later rooting time,moderate stress resistance,and survival rates around 70%,making them as moderately easy to root varieties;Guitang28 have late rooting and high seedling mortality rates,with survival rates at 9.6%,respectively,making them as difficult to root varieties.These varieties with different rooting characteristics provide materials for further studying the mechanism of photosynthetic autotrophic rooting.

关 键 词：甘蔗 组培苗 光合自养生根 品种 

分 类 号：S566.1[农业科学—作物学]