DFI、HDS与实验室精液检验指标相关性及对男性不育诊断价值  

Correlation between DFI,HDS and laboratory semen testing indicators and their diagnostic value for male infertility

作　　者：成金泉 蔡锡金 罗钦泉 李永成 CHENG Jinquan;CAI Xijin;LUO Qinquan;LI Yongcheng(Department of Reproductive Medicine,Luohu District People's Hospital of Shenzhen,Shenzhen,Guangdong 518000,China;Department of Laboratory Medicine,Luohu District People's Hospital of Shenzhen,Shenzhen,Guangdong 518000,China;Department of Laboratory Medicine,Shenzhen Fourth People's Hospital,Shenzhen Sami Medical Center,Shenzhen,Guangdong 518000,China)

机构地区：[1]深圳市罗湖区人民医院生殖医学科,广东深圳518000 [2]深圳市罗湖区人民医院检验科,广东深圳518000 [3]深圳市第四人民医院,深圳市萨米医疗中心检验科,广东深圳518000

出　　处：《医药前沿》2025年第1期82-84,88,共4页Journal of Frontiers of Medicine

摘　　要：目的分析精子DNA碎片指数(DFI)、高DNA着色性(HDS)对男性不育的诊断价值及其与实验室精液检验结果相关性。方法选取2022年1月—2023年1月深圳市罗湖区人民医院及深圳市萨米医疗中心检验科收治的310例男性不育患者作为观察组,另选取同期在上述医疗机构健康体检且结果正常的男性310名作为对照组,对两组进行实验室精液检验[精液体积、精子密度、精子活力、精子正常形态率、精子存活率和前向运动精子比例(PR)],以流式细胞仪和吖啶橙染色法检测DFI、HDS。分析实验室所得检验结果与DFI、HDS的相关性及DFI、HDS对男性不育的诊断价值。结果两组精液体积、精子正常形态率、PR比较,差异无统计学意义(P>0.05);与对照组比较,观察组精子密度、精子活力、精子存活率均更低,差异有统计学意义(P<0.05)。Spearman相关性分析结果显示,DFI与精子活力、精子存活率呈负相关,HDS与精子活力、精子存活率亦呈负相关(P<0.05)。DFI和HDS联合诊断男性不育的价值高于单项诊断。结论男性不育患者的实验室精液检验项目(精子密度、精子活力、精子存活率)均较低,精子质量较差,DFI联合HDS可用于男性不育的辅助诊断。Objective To analyze the diagnostic value of sperm DNA fragment index(DFI)and high DNA staining(HDS)for male infertility and their correlation with laboratory semen test results.Methods Totally 310 male infertility patients admitted to the Laboratory Medicine Department of Luohu District People's Hospital and Shenzhen Sami Medical Center from January 2022 to January 2023 were selected as the observation group,and another 310 male with normal results in the above-mentioned medical structure health examination during the same period were selected as the control group.Laboratory semen tests were conducted on both groups,including semen volume,sperm density,sperm motility,normal sperm morphology rate,sperm survival rate,and progressive motility rate(PR).DFI and HDS were detected by flow cytometry and acridine orange staining.The correlation between the test results obtained from the laboratory and DFI and HDS and the diagnostic value of DFI and HDS for male infertility were analyzed.Results There was no statistically significant difference in semen volume,normal sperm morphology rate,and PR between the two groups(P>0.05).Compared with the control group,the observation group had lower sperm density,sperm motility,and sperm survival rate,and the differences were statistically significant(P<0.05).Spearman correlation analysis showed that DFI were negatively correlated with sperm motility and sperm survival rate and HDS were negatively correlated with sperm motility and sperm survival rate(P<0.05).The combined diagnosis value of DFI and HDS for male infertility was higher than the single diagnosis value.Conclusions The laboratory semen test items(sperm density,sperm motility,sperm survival rate)for male infertility patients are low,and the sperm quality is poor.DFI combined with HDS can be used as an auxiliary diagnosis for male infertility.

关 键 词：实验室精液检验 男性不育 精子体积 精子活力 精子质量 

分 类 号：R817[医药卫生—影像医学与核医学]