AM真菌对镉胁迫下高羊茅生理及镉富集特征的影响  

Effects of AM Fungi on Physiology and Cadmium Enrichment Characteristics of Festuca elata Under Cadmium Stress

作　　者：于丽萍 胡学意 袁雨凡 郭绍霞 徐萌 YU Liping;HU Xueyi;YUAN Yufan;GUO Shaoxia;XU Meng(College of Landscape Architecture and Forestry,Qingdao Agricultural University,Qingdao 266109,China;Binzhou Polytechnic,Binzhou 256603,China)

机构地区：[1]青岛农业大学园林与林学院,山东青岛266109 [2]滨州职业学院,山东滨州256603

出　　处：《青岛农业大学学报(自然科学版)》2024年第4期243-253,258,共12页Journal of Qingdao Agricultural University(Natural Science)

摘　　要：为揭示丛枝菌根(arbuscular mycorrhiza,AM)真菌对高羊茅耐镉(Cd)胁迫的作用,于温室盆栽条件下利用摩西斗管囊霉(Funneliformis mosseae,Fm)进行接种试验,研究了在Cd胁迫下接种AM真菌对高羊茅生长、营养元素积累、抗氧化酶活性、渗透调节物质含量及Cd富集和转运的影响。结果表明:在0~200 mg·kg^(-1)Cd浓度范围内,无论接种AM真菌与否,Cd对高羊茅的株高、地上部干质量、地上部碳(C)、氮(N)、磷(P)和钾(K)积累量存在显著地“低促高抑”效应,在20 mg·kg^(-1)Cd浓度下促进效果最好,接种AM真菌改善了高羊茅的生长及C积累和N、P、K的吸收;与未接种相比,接种AM真菌提高了高羊茅超氧化物歧化酶、过氧化物酶和过氧化氢酶活性,降低了丙二醛含量,且AM真菌接种处理高羊茅的可溶性蛋白、可溶性糖和脯氨酸含量增加,尤其在40 mg·kg^(-1)Cd浓度下效果显著;无论接种AM真菌与否,高羊茅地下部Cd含量和富集量显著高于其地上部,且接种AM真菌增加了植株地上、地下部Cd含量和富集量,植株Cd富集系数在0、20和40 mg·kg^(-1)Cd浓度下分别显著增加29.2%、49.1%和67.9%,即在0~40 mg·kg^(-1)Cd浓度范围内,AM真菌促进高羊茅富集Cd的效果最佳。该研究表明高羊茅-AM真菌共生体对重金属Cd具有较强的富集作用,在Cd污染土壤修复中具有潜在应用价值。In order to reveal the roles of arbuscular mycorrhiza(AM)fungi on cadmium(Cd)stress tolerance in Festuca elata,an inoculation experiment was carried out under greenhouse pot conditions using Funneliformis mosseae(Fm)to investigate the effects of AM fungi inoculation on the growth,nutrient accumulation,antioxidant enzyme activity,osmoregulation substance content,and Cd enrichment and transport of F.elata under Cd stress.The results showed that within the range of 0-200 mg·kg^(-1)Cd concentrations,regardless of inoculation with or without AM fungi,there was a significant"low promotion and high inhibition"effect of Cd on plant height,shoot dry weight,shoot carbon(C),nitrogen(N),phosphorus(P)and potassium(K)accumulation of F.elata,and the best promotion effect was achieved at 20 mg·kg^(-1),and that the AM inoculation improved C accumulation,N,P,K absorption and growth of F.elata.Compared with no inoculation,inoculation with AM fungi increased the activity of superoxide dismutase,peroxidase and catalase and decreased malondialdehyde content in F.elata,and the soluble protein,soluble sugar and proline content of F.elata treated with AM fungi increased,especially at 40 mg·kg^(-1)Cd concentration.Regardless of inoculation with or without AM fungi,the Cd content and enrichment in the roots of F.elata were significantly higher than those in the shoots,and inoculation with AM fungi increased the Cd content and enrichment in shoots and roots.At 0,20,40 mg·kg^(-1)Cd concentrations,Cd bioaccumulation factors of plants were significantly increased by 29.2%,49.1%and 67.9%,respectively,and within the range of 0-40 mg·kg^(-1)Cd concentrations,AM fungi had the best effects on Cd enrichment.This study demonstrates that the F.elata-AM fungal symbiosis has a strong enrichment effect on the heavy metal Cd and has potential application in the remediation of Cd-contaminated soil.

关 键 词：AM真菌 高羊茅 镉 土壤修复 

分 类 号：Q945.79[生物学—植物学]