不同地理分布水松根际微生物群落差异  

Differences in the rhizosphere microbial communities of Glyptostrobus pensilis in different geographical distributions

作　　者：杨晓娟 刘世男[1] 邓必玉 梁永延 YANG Xiaojuan;LIU Shinan;DENG Biyu;LIANG Yongyan(Guangxi Colleges and Universities Key Laboratory for Cultivation and Utilization of Subtropical Forest Plantation,College of Forestry,Guangxi University,Nanning,Guangxi 530004,China;Guangxi Forest Resources and Environment Monitoring Center,Nanning,Guangxi 530028,China;Guangxi Beihai Wetland Ecosystem National Observation and Research Station,Beihai,Guangxi 536000,China)

机构地区：[1]广西大学林学院广西高校亚热带人工林培育与利用重点实验室,广西南宁530004 [2]广西壮族自治区森林资源与生态环境监测中心,广西南宁530028 [3]广西北海湿地生态系统国家定位观测研究站,广西北海536000

出　　处：《森林与环境学报》2025年第1期1-10,共10页Journal of Forest and Environment

基　　金：中央财政林业草原生态保护恢复资金国家重点野生保护植物保护补助项目“广西水松群落资源调查和生态特征研究及迁地保护”(502039202309);广西林业科技推广示范项目“水松根际菌根真菌多样性及促生作用研究”(2024GXLK20)。

摘　　要：为探究水松根际微生物群落特征,以广西野生水松根际土壤为对象,采用高通量测序技术分析不同地理分布水松根际细菌和真菌群落差异,并通过相关性分析和冗余分析探讨其与根际土壤养分和地理气候间的关联。结果显示,广西不同地理分布水松根际微生物优势菌门皆是变形菌门和子囊菌门,优势属皆为大肠杆菌,根际土壤真菌都以腐生型为主。不同地理分布水松根际微生物α多样性指数存在差异,其中,覃塘较低,天等和宾阳较高。相比于其他地理分布的根际微生物,苍梧细菌和雁山真菌生物标志物较多,而覃塘细菌和平乐真菌生物标志物较少。冗余分析结果显示,广西不同地理分布水松土壤养分与根际土壤细菌群落紧密关联,而气候是塑造真菌群落的重要因素。此外,有效磷、全磷、年平均相对湿度、速效钾是细菌群落的驱动因子,年平均相对湿度是真菌的驱动因子。厚壁菌门、酸杆菌门、帕氏菌门、Sva0485、担子菌门和摩氏菌门与土壤磷、钾有紧密关联。To investigate the characteristics of microbial communities in the rhizosphere of Glyptostrobus pensilis,the rhizosphere soil of wild G.pensilis in Guangxi was used as the research subject.High-throughput sequencing technology was used to analyze the differences in bacterial and fungal communities in the rhizosphere of G.pensilis in different geographical distributions.Correlation and redundancy analyses were used to evaluate the relationships among these microbial communities,rhizosphere soil nutrients,and geographical and climatic factors.The results showed that the dominant bacterial and fungal phyla in the rhizosphere soil of G.pensilis across different geographical distributions in Guangxi were Proteobacteria and Ascomycota,respectively,with Escherichia-Shigella being the dominant genus.The fungal communities in the rhizosphere were primarily saprotrophic.There were differences in theα-diversity indices of microbial communities in the rhizosphere soil across different geographical distributions.Compared to other geographical distributions,Qintang showed a relatively lower diversity,whereas Tiandeng and Binyang exhibited a higher diversity.In addition,Cangwu and Yanshan had more bacterial and fungi biomarkers,whereas Qintang and Pingle had fewer bacterial and fungi biomarkers.Redundancy analysis indicated that the soil nutrients in G.pensilis rhizosphere soils from different geographical locations in Guangxi were closely associated with bacterial communities,whereas climate was closely associated with fungal communities.Additionally,available phosphorus,total phosphorus,mean annual relative humidity,and available potassium were identified as the main drivers of bacterial communities,whereas mean annual relative humidity was the main driver of fungal communities.Furthermore,the study revealed that Firmicutes,Acidobacteria,Patescibacteria,Sva0485,Basidiomycota,and Mortierellomycota were closely related to soil phosphorus and potassium levels.

关 键 词：水松 地理分布 根际微生物群落 环境因子 

分 类 号：S154.3[农业科学—土壤学]