甘蓝型油菜亚麻酸含量性状QTL定位与候选基因分析  

QTL Mapping and Candidate Gene Identification for C18:3 Content in Brassica napus L

作　　者：尚丽平 梁佩萱 曹小东 张莉莉 关周博[1] 李殿荣[1] 李保军[1] 王灏[1] SHANG Liping;LIANG Peixuan;CAO Xiaodong;ZHANG Lili;GUAN Zhoubo;LI Dianrong;LI Baojun;WANG Hao(Rapeseed Hybrid Center of Shaanxi Province,Yangling Shaanxi 712100,China)

机构地区：[1]陕西省杂交油菜研究中心,陕西杨凌712100

出　　处：《西北农业学报》2025年第1期24-34,共11页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：陕西省重点研发计划项目(2022NY-159);西藏阿里地区科技计划(QYXTZX-AL2023-03);陕西省‘两链’融合作物育种重点专项(2021LLRH-07-03-01)。

摘　　要：为探究甘蓝型油菜亚麻酸含量性状相关的QTL,以KN群体为试验材料,通过连续7 a的油菜种子亚麻酸含量表型数据,并结合KN高密度遗传连锁图谱采用复合区间作图法对油菜亚麻酸含量QTL进行定位研究。结果表明,共鉴定到72个亚麻酸含量QTL,主要分布在10条染色体上,其中A08染色体上的亚麻酸含量QTL最多有18个,A10和C03染色体上QTL数量次之,均有11个QTL,单个QTL可解释的表型变异范围为2.67%~17.26%。在A08染色体上鉴定到3个亚麻酸含量主效QTL,包括cqC18:3-A8-1、cqC18:3-A8-3和cqC18:3-A8-4。通过对区间内的基因进行基因注释和分析共得到11个亚麻酸含量相关的候选基因。To investigate the quantitative trait loci(QTLs)associated with linolenic acid(C18:3)con-tent in Brassica napus,we utilized the KN population was used as experimental material.A localiza-tion study was conducted using phenotypic data on linolenic acid content from rapeseed seeds collected over seven consecutive years,combined with composite interval mapping and the KN high-density ge-netic linkage map.The analysis identified 72 QTLs influencing linolenic acid content,distributed across 10 chromosomes.The highest concentration was on chromosome A08 with 18 QTLs,followed by 11 QTLs each on chromosomes A10 and C03.The phenotypic variation explained by individual QTLs ranged from 2.67%to 17.26%.Three major QTLs for linolenic acid content were located on chromosome A08,including cqC18:3-A8-1,cqC18:3-A8-3,and cqC18:3-A8-4.Through gene anno-tation and interval analysis,11 candidate genes potentially linked to linolenic acid content were identi-fied.

关 键 词：甘蓝型油菜 双单倍体株系 亚麻酸含量 QTL分析 候选基因鉴定 

分 类 号：S565.4[农业科学—作物学]