不同质量浓度外源ZnONPs对金鱼草耐热性的影响  

Effect of Different Concentrations of Exogenous ZnONPs on the Heat Tolerance of Snapdragon

作　　者：刘丹[1] 毛凤玲 白金鑫[1] LIU Dan;MAO Fengling;BAI Jinxin(Yinchuan University of Energy,Yinchuan 750100,China;School of Agriculture,Ningxia University,Yinchuan 750000,China)

机构地区：[1]银川能源学院,宁夏银川750100 [2]宁夏大学农学院,宁夏银川750000

出　　处：《河南农业科学》2024年第12期139-148,共10页Journal of Henan Agricultural Sciences

基　　金：宁夏回族自治区高等学校科学研究资助项目(NYG2024034);2020年度宁夏回族自治区高校一流本科专业建设项目(高教高办[2021]7号)。

摘　　要：以金鱼草幼苗为试材,探究昼/夜温度25℃/20℃、35℃/30℃、40℃/35℃胁迫下叶面喷施不同质量浓度(0、50、100、200 mg/L)氧化锌纳米颗粒(ZnONPs)对金鱼草幼苗生长、叶绿素含量、渗透调节物质及AsA-GSH循环中酶活性及相关物质的影响,以探明ZnONPs缓解金鱼草热胁迫的作用。结果表明,高温胁迫下金鱼草株高、茎粗、叶长、叶宽以及生物量显著下降,施用不同质量浓度ZnONPs可以缓解金鱼草受到的生长抑制。40℃/35℃胁迫下,与喷施0 mg/L ZnONPs处理相比,喷施50~200 mg/L ZnONPs处理的金鱼草总叶绿素含量和类胡萝卜素含量分别增加3.8%~7.7%和4.9%~11.7%,渗透调节物质可溶性糖含量以及脯氨酸含量分别增加12.7%~44.8%和18.2%~32.9%。40℃/35℃胁迫下,金鱼草As A-GSH循环过程中抗坏血酸过氧化物酶(APX)、脱氢抗坏血酸还原酶(DHAR)、单脱氢抗坏血酸还原酶(MDHAR)及谷胱甘肽还原酶(GR)活性不断降低,喷施不同浓度ZnONPs后金鱼草APX、DHAR、MDHAR以及GR活性分别增加35.3%~86.3%、13.5%~24.6%、58.5%~81.5%和8.6%~19.7%,抗坏血酸(AsA)含量、谷胱甘肽(GSH)含量、抗坏血酸/脱氢抗坏血酸(AsA/DHA)及谷胱甘肽/还原型谷胱甘肽(GSH/GSSG)提高5.9%~11.7%、9.9%~16.5%、14.4%~31.1%和19.5%~37.7%,金鱼草幼苗丙二醛(MDA)含量、相对电导率、超氧阴离子自由基(O_(2)^(-)·)产生速率、过氧化氢(H_(2)O_(2))含量则不断下降。综上,不同ZnONPs处理均能够提高金鱼草耐热性,其中喷施100 mg/L ZnONPs处理提高金鱼草幼苗耐热性的效果最好。Using snapdragon seedlings as test materials,this study investigated the effects of foliar spraying of different concentrations(0,50,100,200 mg/L)of zinc oxide nanoparticles(ZnONPs)on the growth,chlorophyll content,osmotic regulation substances,and enzyme activity and related substances in the AsA-GSH cycle of snapdragon seedlings under day/night temperatures of 25℃/20℃,35℃/30℃,and 40℃/35℃stress,in order to explore the role of ZnONPs in alleviating heat stress in snapdragon seedlings.The results showed that under high temperature stress,the height,stem thickness,leaf length,leaf width,and biomass of snapdragon significantly decreased.Applying different concentrations of ZnONPs could alleviate the growth inhibition of snapdragon.Under 40℃/35℃stress,compared with the treatment of spraying 0 mg/L ZnONPs,the treatment of spraying 50-200 mg/L ZnONPs increased the total chlorophyll content and carotenoid content of snapdragon by 3.8%-7.7%and 4.9%-11.7%,respectively.The soluble sugar content and proline content of osmotic regulating substances increased by 12.7%-44.8%and 18.2%-32.9%,respectively.Under 40℃/35℃ stress,the activities of ascorbate peroxidase(APX),dehydroascorbate reductase(DHAR),monodehydroascorbate reductase(MDHAR),and glutathione reductase(GR)in the AsA-GSH cycle of snapdragon continuously decreased.After spraying different concentrations of ZnONPs,the activities of APX,DHAR,MDHAR,and GR in snapdragon increased by 35.3%-86.3%,13.5%-24.6%,58.5%-81.5%,and 8.6%-19.7%,respectively.The content of ascorbic acid(AsA),glutathion(GSH),ascorbic acid/dehydroascorbate(AsA/DHA),and glutathione/reduced glutathione(GSH/GSSG)increased by 5.9%-11.7%,9.9%-16.5%,14.4%-31.1%,and 19.5%-37.7%,respectively,while the malondialdehyde(MDA)content,relative conductivity,O_(2)^(-)·production rate,and hydrogen peroxide(H_(2)O_(2))content of snapdragon seedlings continued to decrease.The conclusion is that different treatments with ZnONPs can improve the heat tolerance of snapdragon,among which spraying 100 mg/L Zn

关 键 词：金鱼草 高温胁迫 ZnONPs AsA-GSH 叶绿素 活性氧 

分 类 号：S681.9[农业科学—观赏园艺]