姜黄素对糖尿病小鼠肺纤维化的调控作用及机制研究  

Regulatory Effects and Mechanisms of Curcumin on Pulmonary Fibrosis in Diabetic Mice

作　　者：黎双炼 付晨禄 管志威 刘超[2] LI Shuang-lian;FU Chen-lu;LIU Chao(School of Pharmacy,Xianning Medical College,Hubei University of Science and Technology,Xianning Hubei 437100,China)

机构地区：[1]湖北科技学院医学部药学院,湖北咸宁437100 [2]湖北科技学院糖尿病心血管病变湖北省重点实验室

出　　处：《湖北科技学院学报(医学版)》2025年第1期6-9,共4页Journal of Hubei University of Science and Technology(Medical Sciences)

基　　金：咸宁市社发类研发重点专项(2021SFYF006);湖北科技学院糖尿病专项团队项目(2021TNB01)。

摘　　要：目的探讨姜黄素(Cur)对糖尿病(DM)小鼠肺纤维化的改善作用及其机制。方法将50只雄性SPF级C57BL/6小鼠随机分为正常组(Con组,n=10)、姜黄素组(Cur组,n=10)、糖尿病模型组(DM组,n=12)和糖尿病姜黄素处理组(DM+Cur组,n=14)。DM组与DM+Cur组小鼠先给予高糖高脂饲料喂养4周后,小剂量腹腔注射链脲佐菌素[STZ,50mg/(kg·d)]建立糖尿病小鼠模型,1次/d,连续5d,随机血糖16.7mmol/L则为造模成功标准。造模成功后,Cur组与DM+Cur组灌胃给予Cur,200mg/(kg·d),连续16周后处死小鼠,取肺组织,HE染色法、Masson染色及Sirius Red染色观察各组小鼠肺组织病理学改变、炎细胞浸润及胶原沉积情况;Western blot法检测各组小鼠肺组织中TGF-β1、p-Smad3、p-P38MAPK、CollagenⅢ蛋白表达情况。结果与Con组相比,DM组小鼠肺组织呈明显纤维化改变,肺组织中TGF-β1、p-Smad3、p-P38MAPK、CollagenⅢ蛋白表达明显升高(P<0.01)。给予Cur处理后,DM小鼠肺组织纤维化得到明显改善,肺组织中TGF-β1、p-Smad3、p-P38MAPK、CollagenⅢ蛋白表达均有所降低(P<0.05)。结论Cur通过降低纤维化相关蛋白CollagenⅢ的表达明显改善STZ诱导的DM小鼠肺组织纤维化,其作用机制可能与Cur抑制肺组织中TGF-β1/Smad/P38MAPK信号通路激活有关。Objective To investigate the improving effect of curcumin(Cur)on pulmonary fibrosis in diabetic(DM)mice and its mechanism.Methods 50 male SPF grade C57BL/6 mice were randomly divided into a normal group(Con group,n=10),a curcumin group(Cur group,n=10),a diabetic model group(DM group,n=12)and a diabetic curcumin treatment group(DM+Cur group,n=14).The mice in DM group and DM+Cur group were first fed with a high-sugar and high-fat diet for 4 weeks,and then a small dose of streptozotocin[STZ,50mg/(kg·d)]was intraperitoneally injected to establish a diabetic mouse model,once a day for 5 consecutive days.The successful modeling standard was 16.7mmol/L.After successful modeling,the Cur group and the DM+Cur group were gavaged with Cur at 200mg/(kg·d).After 16 consecutive weeks,the mice were killed and the lung tissues were obtained.HE staining,Masson staining and Sirius Red staining were used to observe the pathological changes,inflammatory cell infiltration and collagen deposition in the lung tissues of various groups of mice.Western blot was used to detect the expressions ofTGF-1,p-Smad3、p-P38MAPK and CollagenⅢin the lung tissues of various groups of mice.Results Compared with the Con group,the lung tissue of DM group showed significant fibrosis changes,and the expression of TGF-1,p-Smad3,p-P38MAPK and CollagenⅢproteins in the lung tissue was significantly increased(P<0.01).After Cur treatment,the pulmonary fibrosis of DM mice was significantly improved,and the protein expression of TGF-1,p-Smad3,p-P38MAPK and CollagenⅢin the lung tissue was decreased(P<0.05).Conclusion Cur can significantly improve pulmonary fibrosis in STZ-induced DM mice by reducing the expression of fibrosis-related protein CollagenⅢ,which may be related to the inhibition of TGF-b1/Smad/P38MAPK signaling pathway.

关 键 词：姜黄素 糖尿病肺纤维化 转化生长因子-1 纤维化 

分 类 号：R965[医药卫生—药理学]