Functional metabolomics analysis of the protective mechanism of total flavonoids of Scutellaria baicalensis on acute myocardial ischemia rats  

作　　者：Fang-Ying Tang Ru-Yi Ma An-Yao Xiong Si-Tong Lin Xiao Wang Hong-Jing Dong Jian-Yong Zhang 

机构地区：[1]First Clinical College,Zunyi Medical University,Zunyi 563000,China [2]Shandong Engineering Research Center for Innovation and Application of General Technology for Separation of Natural Products,Shandong Analysis and Test Center,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250014,China [3]Key Laboratory of Basic Pharmacology Ministry Education and Joint International Research Laboratory of Ethnomedicine Ministry of Education,Zunyi Medical University,Zunyi 563000,China

出　　处：《Traditional Medicine Research》2025年第3期21-31,共11页TMR传统医学研究

基　　金：sponsored by Shandong Provincial Key Research and Development Program(Major Technological Innovation Project)([2021]CXGC010508);Guizhou Province Youth Science and Technology Talent Plan(YQK[2023]038);Science and Technology Department of Zunyi City of Guizhou province of China([2020]7);Key project at central government level:the ability establishment of sustainable use for valuable Chinese medicine resources(2060302).

摘　　要：Background:The study aimed to investigate the protective effect and mechanism of total flavonoids of Scutellaria baicalensis(TFSB)on acute myocardial ischemia(AMI)rats by using functional metabonomics.Methods:Rats were divided into the Control,Model,AMI positive control(Propranolol hydrochloride,30 mg/kg),low dose TFSB(50 mg/kg),and high dose TFSB(100 mg/kg)groups.Rats received the corresponding treatment by intragastric administration once daily for 10 consecutive days.Electrocardiogram,myocardial enzyme,triphenyltetrazolium chloride staining,hematoxylin-eosin,and enzyme-linked immunosorbent assay were performed to evaluate the protective effect of TFSB on AMI rats.Then,the UHPLC-Q-Orbitrap MS method based on serum metabolomics was utilised to search for metabolic biomarkers and metabolic pathways.Subsequently,Western blot and RT-PCR techniques were employed to identify the respective genes and proteins.Results:Pharmacodynamics revealed that TFSB could ameliorate AMI in rats.The results of the metabolomics analysis indicated that the alterations in metabolic profile observed in rats with AMI were partially improved by treatment with TFSB.Moreover,the mRNA expression levels of 5-lipoxygenase(5-LOX)and 15-lipoxygenase(15-LOX)and the protein expression levels of 5-LOX,15-LOX,interleukin-1β(IL-1β),and NF-κB p65 were reduced following treatment with TFSB.Conclusion:The potential treatment of TFSB in AMI may be ascribed to its ability to regulate arachidonic acid metabolism.

关 键 词：total flavonoids of Scutellaria baicalensis acute myocardial ischemia liquid chromatography-mass spectrometry functional metabolomics MECHANISM 

分 类 号：R54[医药卫生—心血管疾病]