饲粮中添加过瘤胃氨基酸对湖羊生长性能、血清指标和瘤胃发酵参数的影响  

Effects of rumen amino acids on growth performance,serum indexes, and rumen fermentation parameters of Hu sheep

作　　者：彭洪妹 张建强 张鹏 付陈飞 张治朋 马军德 李元元 张文举[1] PENG Hong-mei;ZHANG Jian-qiang;ZHANG Peng;FU Chen-fei;ZHANG Zhi-peng;MA Jun-de;LI Yuan-yuan;ZHANG Wen-ju

机构地区：[1]石河子大学动物科技学院,新疆石河子832003 [2]新疆巩乃斯种羊场有限公司,新疆伊犁835100

出　　处：《饲料研究》2024年第22期1-6,共6页Feed Research

基　　金：新疆维吾尔自治区重点研发项目(项目编号:2022B02029-2)。

摘　　要：试验旨在研究饲粮中添加过瘤胃赖氨酸(RPLys)和过瘤胃蛋氨酸(RPMet)对育肥湖羊生长性能、血清生化指标、抗氧化能力、免疫性能和瘤胃发酵参数的影响。试验选取健康、体重(20.22±4.01) kg、3月龄的育肥湖羊(公羊) 32只,随机分为4组,每组8个重复,每个重复1只羊。对照组饲喂基础饲粮,试验组分别在基础饲粮中添加0.15%RPLys+0.05%RPMet (试验Ⅰ组)、0.45%RPLys+0.15%RPMet (试验Ⅱ组)、0.75%RPLys+0.25%RPMet (试验Ⅲ组)。预试期7 d,正式试验期52 d。结果显示:各组湖羊平均日增重、平均日干物质采食量、料重比均差异不显著(P>0.05);试验Ⅰ组白球比(A/G)显著高于对照组和试验Ⅱ组(P<0.05);与对照组相比,各试验组血清碱性磷酸酶(ALP)活性、总抗氧化能力(T-AOC)显著升高(P<0.05),各试验组丙二醛(MDA)含量显著降低(P<0.05)。试验Ⅱ组血清谷胱甘肽过氧化物酶(GSH-Px)活性显著高于其他组(P<0.05),试验Ⅱ组和试验Ⅲ组血清过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活性显著高于对照组和试验Ⅰ组(P<0.05)。与对照组相比,各试验组血清免疫球蛋白A (IgA)、免疫球蛋白G (IgG)、免疫球蛋白M(IgM)含量显著提高(P<0.05),试验Ⅱ组和试验Ⅲ组血清白细胞介素-2 (IL-2)含量显著下降(P<0.05)。研究表明,饲粮中添加RPLys和RPMet能够增强湖羊免疫性能和抗氧化能力,不会对血清生化指标和瘤胃发酵产生负面影响,在饲粮中添加0.45%RPLys和0.15%RPMet效果较好。The study aimed to explore the effects of rumen lysine(RPLys)and rumen methionine(RPMet)on the growth performance,serum biochemical indexes,antioxidant capacity,immune performance and rumen fermentation parameters in the basic diet of fattening Hu sheep.Thirty-two healthy three-month-old fattened Hu sheep(rams)with the body weight of(20.22±4.01)kg were randomly divided into four groups with eight replicates per group and one sheep per replicate.The control group was fed a basal diet,experimental groups were fed the basal diet supplemented with 0.15%RPLys+0.05%RPMet(trial groupⅠ),0.45%RPLys+0.15%RPMet(trial groupⅡ)and 0.75%RPLys+0.25%RPMet(trial groupⅢ),respectively.The pre-test period was seven days,and the formal test period was 52 days.The results showed that there were no significant differences in average daily gain,average daily dry matter intake and ratio of feed to gain of Hu sheep among all groups(P>0.05).The A/G in trial groupⅠwas significantly higher than that in control group and trial groupⅡ(P<0.05).Compared with the control group,the serum alkaline phosphatase(ALP)activity and total antioxidant capacity(T-AOC)in trial groups were significantly increased(P<0.05),and the malondialdehyde(MDA)content in experimental groups was significantly decreased(P<0.05).The serum glutathione peroxidase(GSH-Px)activity of trial groupⅡwas significantly higher than that of the other groups(P<0.05),and the catalase(CAT)and superoxide dismutase(SOD)activities of trial groupsⅡandⅢwere significantly higher than those of control group and trial groupⅠ(P<0.05).Compared with the control group,the contents of serum immunoglobulin A(IgA),immunoglobulin G(IgG)and immunoglobulin M(IgM)in trial groups were significantly increased(P<0.05),and the content of interleukin-2(IL-2)in trial groupsⅡandⅢwas significantly decreased(P<0.05).The study indicates that dietary supplementation of RPLys and RPMet can enhance the immune performance and antioxidant capacity of Hu sheep,but has no negative effects on serum bioc

关 键 词：过瘤胃赖氨酸 过瘤胃蛋氨酸 湖羊 血清指标 瘤胃发酵参数 

分 类 号：S826[农业科学—畜牧学]