中药复方对绵羊瘤胃发酵参数、消化酶及微生物组成的影响  

Effects of Chinese herbal compound on rumen fermentation parameters,digestive enzymes,and microbial composition of sheep

作　　者：韩怡静 何振莲 于天力 任靖宇 裴彩霞[1] HAN Yi-jing;HE Zhen-lian;YU Tian-li;REN Jing-yu;PEI Cai-xia

机构地区：[1]山西农业大学动物科学学院,山西晋中030801

出　　处：《饲料研究》2024年第22期7-11,共5页Feed Research

基　　金：国家自然科学基金项目(项目编号:31972590);山西省自然科学研究面上项目(项目编号:202303021221096)。

摘　　要：试验旨在探究中药复方对绵羊瘤胃发酵参数、消化酶及微生物组成的影响。试验选取30只体重(20.81±1.27) kg的3月龄杜寒杂交公羔,随机分为3组,每组10个重复,每个重复1只羊。各组中药复方添加量为0 (对照组)、10 (Z1组)、30 g/d (Z3组)。预试期15 d,正式试验期50 d。结果显示,与对照组相比,中药复方组的氨态氮含量、乙酸/丙酸显著降低(P<0.05);总挥发性脂肪酸、丙酸以及微生物蛋白含量均显著提高(P<0.05)。Z3组的总挥发性脂肪酸、丙酸的含量显著高于Z1组(P<0.05),乙酸含量显著高于Z1组及对照组(P<0.05)。Z3组的纤维二糖酶活性显著高于对照组(P<0.05);Z2组、Z3组的果胶酶和α-淀粉酶活性均显著高于对照组(P<0.05);中药复方组的蛋白酶活性显著低于对照组(P<0.05)。Z1组、Z3组总细菌数量均显著高于对照组(P<0.05),且Z3组总细菌数量显著高于Z1组(P<0.05);对照组总产甲烷菌数量显著高于Z3组(P<0.05);Z3组黄色瘤胃球菌、溶纤维丁酸弧菌、嗜淀粉瘤胃杆菌数量均显著高于对照组和Z1组(P<0.05)。研究表明,饲粮中补充中药复方有利于改善绵羊消化性能及瘤胃微生物的组成,且适宜添加量为30 g/d。The purpose of this experiment was to investigate the effects of Chinese herbal compound on rumen fermentation parameters,digestive enzymes,and microbial composition of sheep.Thirty three-month-old male Dorper×Small-tailed Han crossbred lambs with body weight of(20.81±1.27)kg were randomly divided into three groups with 10 replicates in each group and each replicate consisting of one lamb.The dosage of Chinese herbal compound was 0(control group),10(Z1 group)and 30 g/d(Z3 group).The preliminary period lasted for 15 days,and the formal experimental period lasted for 50 days.The results showed that compared with the control group,the ammonia nitrogen content and the ratio of acetic acid to propionic acid in the Chinese herb compound group were significantly lower(P<0.05).The concentrations of total volatile fatty acids,propionic acid,and microbial protein were significantly increased(P<0.05).The concentrations of total volatile fatty acids and propionic acid in the Z3 group were significantly higher than that in the Z1 group(P<0.05).Acetic acid in Z3 group was significantly higher than that in Z1 group and control group(P<0.05).The cellulase activity in the Z3 group was significantly higher than that in the control group(P<0.05).The pectinase andα-amylase activities in the Z2 and Z3 groups were significantly higher than those in the control group(P<0.05).The protease activity in the Chinese herbal compound group was significantly lower than that in the control group(P<0.05).The total bacterial count in Z1 and Z3 groups were significantly greater than that of the control group(P<0.05),the Z3 group was significantly higher than that in Z1 group(P<0.05).The total number of methanogens in the control group was significantly greater than that in Z3 group(P<0.05).The Ruminococcusflavefaciens,Butyrivibrio fibrisolvens,and Ruminobacter amylophilus in Z3 group were significantly higher than that in control group and Z1 group(P<0.05).The results show that the supplementation of Chinese herbal compound in the diet of sheep

关 键 词：中草复方 绵羊 瘤胃发酵参数 消化酶 微生物组成 

分 类 号：S826[农业科学—畜牧学]