柚果多糖对斜带石斑鱼脂肪沉积和肝脏健康的影响  

Effects of pomelo fruit polysaccharide on fat deposition and liver health of Epinephelus coioides

作　　者：张潇潇 孙梓祯 邬颖欣 刘祎帆 白卫东 吴锦辉 邹翠云 ZHANG Xiao-xiao;SUN Zi-zhen;WU Ying-xin;LIU Yi-fan;BAI Wei-dong;WU Jin-hui;ZOU Cui-yun

机构地区：[1]仲恺农业工程学院动物科技学院,广东广州510225 [2]广东省农业技术推广中心,广东广州510520

出　　处：《饲料研究》2024年第22期52-58,共7页Feed Research

基　　金：广东省科技创新战略专项资金(项目编号:pdjh2022a0247);大学生创新创业训练计划项目(项目编号:S202311347007);广东省重点建设学科科研能力提升项目(项目编号:2022ZDJS022);广东茂名滨海新区海洋渔业产业园-现代渔业全产业链技术研究项目(项目编号:0835-220FA8102621)。

摘　　要：试验旨在探究柚果多糖对降低斜带石斑鱼(Epinephelus coioides)脂肪沉积和改善肝脏健康的作用。选取体重为11 g左右的斜带石斑鱼,随机分为5组,分别为对照组(脂肪含量11.06%)、低脂组(脂肪含量7.49%)、低脂+柚果多糖组(低脂饲料+400 mg/kg柚果多糖)、高脂组(脂肪含量15.32%)和高脂+柚果多糖组(高脂饲料+400 mg/kg柚果多糖),每组3个重复,每个重复20尾鱼。预试期2 w,正式试验期8 w。结果显示,低脂组斜带石斑鱼的增重率(WGR)和特定生长率(SGR)显著低于对照组(P<0.05),低脂+柚果多糖组和高脂+柚果多糖组分别高于低脂组和高脂组(P>0.05)。高脂+柚果多糖组与高脂组相比,全鱼和肌肉粗脂肪含量、肝体比(HSI)显著降低(P<0.05)。低脂组和高脂组出现肝损伤,低脂+柚果多糖组和高脂+柚果多糖组对肝组织结构损伤具有明显的改善作用,且高脂+柚果多糖组中肝脏脂滴含量较高脂组明显下降。高脂+柚果多糖组可显著抑制炎症和凋亡相关基因白细胞介素-1β (IL-1β)、白细胞介素-8 (IL-8)、半胱天冬氨酸蛋白酶-3 (caspase-3)和半胱天冬氨酸蛋白酶-8 (caspase-8)的上调(P<0.05)。研究表明,在低脂和高脂饲料中添加柚果多糖对斜带石斑鱼生长性能无影响,但可以降低高脂饲料引起的全鱼和肌肉中粗脂肪含量升高及肝脏中脂肪沉积,增强免疫功能,改善肝脏健康。The experiment aimed to investigate the effects of pomelo fruit polysaccharide on reducing fat deposition and improving liver health in Epinepheluscoioides.Selected Epinepheluscoioideswith a body weight of about 11 g,divided into five groups.There were three replicates in each group:Control group(fat content 11.06%),low-fat group(fat content 7.49%),low-fat+pomelo fruit polysaccharide group(low-fat diet+400 mg/kg pomelo fruit polysaccharide),high-fat group(fat content 15.32%)and high-fat+pomelo fruit polysaccharide group(high-fat diet+400 mg/kg pomelo fruit polysaccharide).20 fish per repeat.The pre-test period was two weeks,the formal test period was eight weeks.The results showed that the weight gain rate(WGR)and specific growth rate(SGR)of Epinepheluscoioides in the low-fat group were significantly lower than those in the control group(P<0.05).The low-fat+pomelo fruit polysaccharide group and the high-fat+pomelo fruit polysaccharide group were higher than the low-fat group and the high-fat group,respectively,but without significant differences(P>0.05).Compared to the high-fat group,the whole fish and muscle crude fat content and hepatosomatic index(HSI)in the high-fat+pomelo fruit polysaccharide group were significantly reduced(P<0.05).The low-fat group and high-fat group showed liver damage,but the low-fat+pomelo fruit polysaccharide group and the high-fat+pomelo fruit polysaccharide group exhibited significant improvements in liver tissue structure damage,and the liver lipid droplet content in the high-fat+pomelo fruit polysaccharide group was significantly lower than that in the high-fat group.The high-fat+pomelo fruit polysaccharide group significantly inhibited the upregulation of inflammation and apoptosis-related genes interleukin-1β(IL-1β),interleukin-8(IL-8),caspase protease-3(caspase-3),and caspase protease-8(caspase-8).The study indicates that the addition of pomelo polysaccharide in low fat and high fat diets had no effect on the growth performance of Epinephelus coioides,but it could reduce the i

关 键 词：柚果多糖 斜带石斑鱼 肝损伤 脂肪沉积 免疫功能 

分 类 号：S963.7[农业科学—水产养殖]