基于16S rDNA测序技术探讨Pony马及蒙古马肠道菌群的差异  

A comparative analysis of gut microbiome variations between Pony horses and Mongolian horses utilizing 16S rDNA sequencing technology

作　　者：钟伟国 刘荣启 韩银涛 陈杰 蓝荣彬 王瑞深 王润良 廖建昭 ZHONG Wei-guo;LIU Rong-qi;HAN Yin-tao;CHEN Jie;LAN Rong-bin;WANG Rui-shen;WANG Run-liang;LIAO Jian-zhao

机构地区：[1]深圳市农产品质量安全检验检测中心深圳市动植物疫病预防控制中心,广东深圳518107 [2]华南农业大学兽医学院,广东广州510642

出　　处：《饲料研究》2024年第22期78-83,共6页Feed Research

基　　金：华南农业大学特定高校学科建设专项(项目编号:2023B10564003);深圳市质量安全检验检测研究院自选课题(项目编号:NJ-FW-20240045)。

摘　　要：试验旨在探讨Pony马和蒙古马之间的肠道微生物群组成的差异。试验从Pony和蒙古马的粪便样本中提取脱氧核糖核酸(DNA),采用多样性分析、分类学分析和差异分析等方法研究二者间肠道菌群的差异。结果显示,Pony马和蒙古马的肠道菌群在门水平上无显著差异(P>0.05),在属水平上,蒙古马肠道UCG-004和拉梅尔氏芽孢杆菌属相对丰度显著高于Pony马(P<0.05)。功能预测分析显示,Pony马和蒙古马肠道差异微生物群富集在广泛特异性磷酸酶磷酸孔蛋白(PhoE)、冷休克蛋白和3-氧酰基-酰基载体蛋白合成酶的信号通路上。蒙古马血清中干扰素-α (IFN-α)、干扰素-β (IFN-β)、干扰素-γ (IFN-γ)、肿瘤坏死因子-α (TNF-α)和白细胞介素-6 (IL-6)的mRNA表达水平均显著或极显著高于Pony马(P<0.05或P<0.01)。研究表明,Pony马和蒙古马之间的肠道微生物群组成存在显著差异,拉梅尔氏芽孢杆菌属可能通过调节特异性磷酸酶PhoE相关信号通路来影响Pony和蒙古马先天免疫功能,使蒙古马的免疫力高于Pony马。The study aimed to investigate the differences in gut microbiome composition between Pony horses and Mongolian horses.Fecal samples from Pony and Mongolian horses were collected,and deoxyribonucleic acid(DNA)was extracted to study the differences in gut microbiota between the two using diversity analysis,taxonomic analysis,and differential analysis methods.The results showed that there were no significant differences in the gut microbiota of Pony horses and Mongolian horses at the phylum level(P>0.05),but at the genus level,the relative abundance of UCG-004 and Rummeliibacilluswas significantly higher in the Mongolian horse than in the Pony horse(P<0.05).Functional prediction analysis revealed that the differential gut microbiota of Pony horses and Mongolian horses were enriched in pathways related to broad-specificity phosphatase PhoE,cold shock proteins,and 3-oxoacyl-acyl carrier protein synthase.The mRNA expression levels of interferon-α(IFN-α),interferon-β(IFN-β),interferon-γ(IFN-γ),tumor necrosis factor-α(TNF-α),and interleukin-6(IL-6)in the serum of Mongolian horses were all significantly or extremely higher than those in Pony horses(P<0.05 or P<0.01).The study indicates that there are significant differences in the composition of the gut microbiota between Pony horses and Mongolian horses,and Rummeliibacillus may affect the innate immune function of Pony and Mongolian horses by regulating the signal pathway related to specific phosphatase PhoE.This may result in Mongolian horses having a higher level of immunity than Pony horses.

关 键 词：Pony马 蒙古马 肠道菌群 拉梅尔氏芽孢杆菌属 

分 类 号：S821[农业科学—畜牧学]