A-FABP基因在三个品种鸡中的群体遗传多态性分析  

Analysis of population genetic polymorphism of the A-FABP gene in three varieties of chicken

作　　者：陈芝媛 苏咏梅[1] 朱文进[1] 宋雪柔 张铷铄 代晓琳 CHEN Zhi-yuan;SU Yong-mei;ZHU Wen-jin;SONG Xue-rou;ZHANG Ru-shuo;DAI Xiao-lin

机构地区：[1]河北科技师范学院动物科技学院,河北秦皇岛066004

出　　处：《饲料研究》2024年第23期113-118,共6页Feed Research

基　　金：秦皇岛市科学技术研究与发展计划项目(项目编号:201803B011);秦皇岛市市级农业科技成果转化基金项目(项目编号:2014);鸡现代种业科技创新团队(项目编号:21326303D)。

摘　　要：试验旨在探究脂肪细胞型脂肪酸结合蛋白(A-FABP)基因在坝上长尾鸡、东乡黑鸡和白羽来航鸡3个品种中的遗传多态性。试验采用单链构象多态性分析(PCR-SSCP)方法对3个品种鸡的A-FABP基因进行SNP检测。结果显示,共发现4个突变位点:g.09A>G、g.110T>C、g.1607A>G和g.1727A>C,均为在鸡中首次发现的突变位点,且g.1607A>G和g.1727A>C两个位点的突变只发生在坝上长尾鸡。坝上长尾鸡多态信息含量(PIC平均值为0.3280)和观测杂合度(Ho平均值为0.5945)在3个品种中均为最高。Hardy-Weinberg平衡性检验发现坝上长尾鸡有3个突变位点(g.09A>G、g.1607A>G和g.1727A>C)处于平衡状态(P>0.05),白羽来航鸡的2个突变位点(g.09A>G和g.110T>C)处于平衡状态(P>0.05),其余各位点处于不平衡状态(P<0.05)。研究表明,A-FABP基因在坝上长尾鸡的遗传多态性高于东乡黑鸡和白羽来航鸡。The experiment aimed to investigate the population genetic polymorphism of fat adipocyte type fatty acid binding protein(A-FABP)gene among Bashang long-tail chicken,Dongxiang black chicken,and Baiyu Leghorn chicken.The SNP detection of A-FABP gene sequences in different breeds were carried out by the PCR-SSCP method.The results showed that there were four mutation sites:g.09A>G,g.110T>C,g.1607A>G,and g.1727A>C,all of which were first discovered in chickens.The mutations at the two loci of g.1607A>G and g.1727A>C occurred only in the Bashang long-tail chicken,and Bashang long-tail chickens had the highest polymorphic information content(mean PIC value 0.3280),and the highest observed heterozygosity(average Ho 0.5945).Hardy-Weinberg equilibrium test found that three mutation sites(g.09A>G,g.1607A>G and g.1727A>C)in Bashang long-tail chickens were in equilibrium(P>0.05),and two mutation sites(g.09A>G,g.110T>C)in Baiyu Leghorn chicken were in equilibrium.(P>0.05),and the other sites are in an unbalanced state(P<0.05).The study indicates that the genetic polymorphism of A-FABP gene in Bashang long-tailed chicken was higher than that in Dongxiang black chicken and Baiyu Leghorn chicken.

关 键 词：地方品种鸡 A-FABP PCR-SSCP 群体遗传多态性 

分 类 号：S831.2[农业科学—畜牧学]