分散固相萃取净化结合气相色谱-三重四极杆串联质谱法同时测定宠物食品中24种调味剂  

Simultaneous determination of 24 flavors in pet food by dispersive solid-phase extraction purification combined with gas chromatography-triple quadrupole mass spectrometry

作　　者：方丽 周漪波 王李平 江子荣 黄嘉颖 叶绮云 霍柱健 李容梅 FANG Li;ZHOU Yi-bo;WANG Li-ping;JIANG Zi-rong;HUANG Jia-ying;YE Qi-yun;HUO Zhu-jian;LI Rong-mei

机构地区：[1]广东省科学院测试分析研究所(中国广州分析测试中心)、广东省化学测量与应急检测技术重点实验室,广东省保健食品功效成分检测与风险物质快速筛查工程技术研究中心,广东广州510070 [2]天地壹号饮料股份有限公司,广东江门529075

出　　处：《饲料研究》2024年第23期140-147,共8页Feed Research

基　　金：江门市科技计划项目(项目编号:江科[2021]182号)。

摘　　要：试验旨在建立分散固相萃取净化结合气相色谱-三重四极杆串联质谱法同时测定宠物食品中24种调味剂含量的方法。样品经乙酸乙酯超声提取,十八烷基键合硅胶(C_(18))、石墨化炭黑(GCB)和无水硫酸镁(MgSO_(4))净化,DB-1701色谱柱分离,多反应监测(MRM)采集,基质匹配外标法定量。结果显示,24种调味剂线性关系良好,相关系数(r^(2))>0.9960,检出限和定量限范围为0.010~0.511 mg/kg和0.035~1.704 mg/kg,在低、中、高3个浓度水平的加标回收率为72.3%~115.3%,相对标准偏差为0.6%~10.7%(n=6)。研究表明,试验方法前处理操作简单、灵敏度高、方法稳定、抗干扰性强,能够为宠物食品中调味剂的分析测定提供依据。The experiment aimed to establish a method for simultaneous determination of 24 flavors in pet food using dispersive solid-phase extraction(SPE)purification followed by gas chromatography-triple quadrupole mass spectrometry(GC-MS/MS).Samples are extracted using ethyl acetate by ultrasonication and purified using C_(18),graphitized carbon black(GCB)and MgSO_(4).Separation was achieved using a DB-1701 chromatographic column,and quantification was performed using the matrix-matched external standard method with multiple reaction monitoring(MRM).The results showed that the 24 flavoring agents had a good linear relationship with correlation coefficients(r^(2)>0.9960),the ranges of limits of detection and limits of quantitation were expressed in 0.010~0.511 mg/kg and 0.035~1.704 mg/kg,respectively.The recovery rates at three concentration levels(low,medium and high)are 72.3%~115.3%,with relative standard deviations of 0.6%~10.7%(n=6).The study indicates that the experimental method has simple sample preparation steps,high sensitivity,method stability,and strong resistance to interference,providing a basis for the analysis and determination of flavors in pet food.

关 键 词：宠物食品 调味剂 分散固相萃取净化 气相色谱-三重四极杆串联质谱法 基质效应 

分 类 号：S816.17[农业科学—饲料科学]