球毛壳菌ND35对盐胁迫下辣椒幼苗生长和生理特性的影响  

Effects of Chaetomium globosum ND35 on Growth and Physiological Characteristics of Pepper Seedlings under Salt Stress

作　　者：孟庆果 李玲 高广增 孙帅斌 闫学成 高克祥 Meng Qingguo;Li Ling;Gao Guangzeng;Sun Shuaibin;Yan Xuecheng;Gao Kexiang(Trading Center for Public Resources of Yishui County,Yishui 276400,China;College of Plant Protection,Shandong Agricultural University/Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests,Taian 271018,China;Feicheng Bureau of Agriculture and Rural Affairs,Feicheng 271600,China;Qingdao Forestry Plant Hospital Engineering Co.,Ltd.,Qingdao 266100,China)

机构地区：[1]沂水县公共资源交易中心,山东沂水276400 [2]山东农业大学植物保护学院/山东省蔬菜病虫生物学重点实验室,山东泰安271018 [3]肥城市农业农村局,山东肥城271600 [4]青岛林业植物医院工程有限公司,山东青岛266100

出　　处：《山东农业科学》2024年第12期47-53,共7页Shandong Agricultural Sciences

基　　金：山东省重点研发计划项目(2018GNC111005);山东省重大科技创新工程项目(2019JZZY010714)。

摘　　要：为给球毛壳菌(Chaetomium globosum)ND35在盐碱地蔬菜生产中的应用提供技术依据,本试验以辣椒幼苗材料,采用浇灌不同浓度盐溶液(0、50、100、150 mmol·L^(-1)NaCl)方法模拟盐胁迫,研究接种内生真菌球毛壳菌ND35对盐胁迫下辣椒幼苗生长和生理特性的影响。结果表明:盐胁迫下,与不接种球毛壳菌ND35的处理(CK)相比,接种球毛壳菌ND35能提高辣椒幼苗地上部、地下部鲜重及根冠比;0、50、100、150 mmol·L^(-1)NaCl胁迫下接种球毛壳菌ND35处理辣椒叶片的可溶性蛋白含量较CK分别提高16.05%、9.11%、17.51%和6.38%,丙二醛含量分别降低15.59%、6.68%、13.68%和18.02%,脯氨酸含量分别降低26.93%、36.48%、33.28%和50.80%,抗氧化酶(SOD、POD、CAT)活性都高于CK,且NaCl浓度为150 mmol·L^(-1)时辣椒叶片的SOD活性比CK提高126.86%。综之,接种内生真菌球毛壳菌ND35能够提高盐胁迫下辣椒幼苗的耐受性,保护其健康生长。In order to provide technical basis for the application of Chaetomium globosum ND35 in pro⁃duction of vegetables in saline⁃alkali soil,pepper seedlings were used as materials in the test,and the effects of inoculation with endophytic fungus C.globosum ND35 on growth and physiological characteristics of pepper seedlings under salt stress were studied by irrigating different salt solution(0,50,100 and 150 mmol·L^(-1)NaCl)to simulate salt stress.The results showed that under salt stress,inoculation with C.globosum ND35 could improve the fresh weight above and under ground as well root⁃shoot ratio of pepper seedlings compared with the control(CK)without inoculation.Under stress of 0,50,100 and 150 mmol·L^(-1)NaCl,the soluble protein content in leaves inoculated with C.globosum ND35 increased by 16.05%,9.11%,17.51%and 6.38%,respectively compared with CK;the malondialdehyde(MDA)and proline(Pro)contents decreased by 15.59%,6.68%,13.68%and 18.02%,and 26.93%,36.48%,33.28%and 50.80%,respectively;the activities of antioxidant enzymes(CAT,POD,SOD)were higher than that of CK,and the SOD activity in⁃creased by 126.86%when the NaCl concentration was 150 mmol·L^(-1).In conclusion,inoculation with the en⁃dophytic fungus C.globosum ND35 could enhance tolerance of pepper seedlings under salt stress and protect their healthy growth.

关 键 词：内生真菌 球毛壳菌ND35 辣椒 盐胁迫 生理特性 

分 类 号：S641.3[农业科学—蔬菜学]