黑龙江齐齐哈尔地区牛呼吸道疾病综合征重要病原体流行病学调查  

Epidemiological investigation of important pathogens of bovine re respiratory disease syndrome in Qiqihar spiratory Qiqihar,Heilongjiang Province

作　　者：王秀东 李娜 WANG Xiudong;LI Na(Panjin Inspection and Testing Center,Panjin 124000,China;Heilongjiang Fire Eye Testing Technology Co.,Ltd,Qiqihar 161005,China)

机构地区：[1]盘锦检验检测中心,辽宁盘锦124000 [2]黑龙江火眼检测技术有限公司,黑龙江齐齐哈尔161005

出　　处：《养殖与饲料》2025年第1期46-51,共6页Animals Breeding and Feed

摘　　要：[目的]了解黑龙江省齐齐哈尔地区牛呼吸道疾病综合征重要病原体流行与病原菌的耐药情况,为齐齐哈尔地区牛呼吸道疾病综合征的防治提供依据。[方法]采集齐齐哈尔不同地区规模化养殖场及散养户发病牛的鼻拭子样本594份,应用PCR检测样本中携带的牛呼吸道疾病综合征病原体,并进行细菌分离培养及药敏试验。[结果]594份鼻拭子样品中共有209份样品携带牛呼吸道疾病综合征病原体,其中牛支原体检出率为33.01%、多杀性巴氏杆菌检出率为15.79%、溶血性曼氏杆菌检出率为16.75%、牛传染性鼻气管炎病毒检出率为15.79%、牛病毒性腹泻病毒检出率为20.57%。通过细菌分离培养获得多杀性巴氏杆菌27株、溶血性曼氏杆菌26株。通过药敏试验发现多杀性巴氏杆菌和溶血性曼氏杆菌三重耐药菌株占比分别为51.85%和38.46%,四重耐药菌株占比分别为22.22%和23.08%,五重耐药菌株占比分别为22.22%和15.38%,六重耐药菌株占比分别为3.70%和23.08%。[结论]黑龙江省齐齐哈尔地区引起牛呼吸道疾病综合征的重要病原体检出率较高,多杀性巴氏杆菌和溶血性曼氏杆菌菌株多重耐药情况较为严重。[Objectives]The prevalence and drug resistance of important pathogens of bovine respiratory disease syndrome in Qiqihar,Heilongjiang Province was investigated to provide a basis for the prevention and treatment of bovine respiratory disease syndrome in Qiqihar.[Methods]594 nasal swab samples of diseased cattle were collected from large-scale farms and free-range households in different areas of Qiqihar.PCR was used to detect the pathogens of bovine respiratory disease syndrome carried in the sample.The isolation,identi‐fication,and the drug sensitivity test of bacteria were conducted.[Results]209 nasal swab samples out of the 594 collected samples carried the pathogen of bovine respiratory syndrome,with a detection rate of 33.01%,15.79%,16.75%,15.79%,and 20.57%for Mycoplasma bovis,Pasteurella multocida,Mannheimia haemolytica,infectious bovine rhinortracheitis virus,and bovine viral diarrhea virus,respectively.27 Pasteurella multocida strains and 26 Mannheimia haemolytica strains were obtained through the isolation and cultivation of bacteria.The results of the drug sensitivity test showed that the proportion of triple,quadruple,penta-plex and hexaplex resistant strains of Pasteurella multocida and Mannheimia haemolytica was 51.85%and 38.46%,22.22%and 23.08%,22.22%and 15.38%,3.70%and 23.08%,respectively.[Conclusions]The detection rate of important pathogens of bovine respiratory disease syndrome in Qiqihar,Heilongjiang Province was relatively high,and the multi-drug resistance to the Pasteurella multocida and Mannheimia haemolytica strain was particularly se‐vere.

关 键 词：牛呼吸道疾病综合征 病原体 细菌分离鉴定 PCR检测 药敏试验 

分 类 号：S858.23[农业科学—临床兽医学]