流式细胞术鉴定海带孢子体倍性的研究  

Research on Identifying the Ploidy of Saccharina Japonica Sporophyte Using Flow Cytometry

作　　者：田萍萍 李言 刘延岭 李晓捷 王伟伟 陈书秀 TIAN Pingping;LI Yan;LIU Yanling;LI Xiaojie;WANG Weiwei;CHEN Shuxiu(Shandong Oriental Ocean Sci-tech Co.Ltd,Yantai,Shandong 264003,China;Shandong Technology Innovation Center of Algae and Sea Cucumber,Yantai,Shandong 264003,China;Provincial Key Laboratory of Marine Seed Industry of Shandong,Yantai,Shandong 264003,China)

机构地区：[1]山东东方海洋科技股份有限公司,山东烟台264003 [2]山东省海水种业重点实验室,山东烟台264003 [3]山东省海藻与海参技术创新中心,山东烟台264003

出　　处：《水产养殖》2025年第1期17-20,共4页Journal of Aquaculture

基　　金：山东省重点研发计划(重大科技创新工程)项目(2022LZGC004);国家藻类产业技术体系(CARS-50);国家重点研发计划“蓝色粮仓科技创新”重点专项(2018YFD0901500);山东省农业良种工程项目(2021LZGC004);山东省现代农业藻类产业技术体系项目(SDAIT-26)。

摘　　要：为满足海带倍性育种中对多倍体海带倍性的鉴定需要,以海上栽培3~5个月的海带孢子体为材料,制备并收集原生质体。结果表明,海带孢子体生长部组织酶解后,得到2种直径大小不同的原生质体,满足流式细胞仪检测DNA含量检测倍性所需的原生质体数量要求。海带孢子体原生质体经细胞核解离液解离,于流式细胞仪检测后,获得了可用于估测海带DNA含量鉴定海带孢子体倍性的流式直方图。表明流式细胞术鉴定海带孢子体倍性的方法,适用于海带孢子体倍性的初步鉴定。The protoplasts of Saccharina japonica were prepared and collected from kelp sporophytes which were cultivated at sea for 3-5months to address the identification needs in kelp polyploidy breeding.The result showed that two types of protoplasts with different diameters were obtained after enzymatic hydrolysis of the growth tissue of kelp sporophytes,which meet the requirement for DNA content and ploidy detection by Flow Cytometry.Then,they were tested using Flow Cytometry after cell dissociation and fluorescence staining.A Flow Cytometry histogram would be obtained that can be used to estimate DNA content and then identify the ploidy of S.japonica sporophyte.The research has indicated that the Flow Cytometry method for identifying the ploidy is suitable for preliminary ploidy identification of kelp sporophytes.

关 键 词：流式细胞仪 海带 孢子体 倍性鉴定 原生质体 

分 类 号：S968.4[农业科学—水产养殖]