METTL3在小鼠根尖牙乳头干细胞增殖及迁移中的作用  

he role of METTL3 in the proliferation and migration of murine apical dental papilla stem cells

作　　者：朱永娜 谢智鹏 吴越 何泽禹 葛翔 ZHU Yong-Na;XIE Zhi-Peng;WU Yue(The Second Affiliated Hospital of Bengbu Medical University,Bengbu 233000,Anhui,China)

机构地区：[1]蚌埠医科大学第二附属医院,安徽蚌埠233000 [2]蚌埠医科大学

出　　处：《吉林医学》2025年第1期9-13,共5页Jilin Medical Journal

基　　金：安徽省高校自然科学研究项目[项目编号:2023AH051997]。

摘　　要：目的:探索METTL3对小鼠根尖牙乳头干细胞(SCAPs)增殖及迁移的调节作用。方法:分离培养小鼠SCAPs;流式细胞术鉴定干细胞表面标志分子;成骨诱导/成脂诱导后鉴定多项分化潜能;shRNA干预SCAPs中METTL3表达,并用QPCR鉴定干预效果;敲除SCAPs中的METTL3后,CCK-8分析其增殖能力变化,跨孔实验及划痕实验检测迁移能力的改变。结果:SCAPs高表达间充质干细胞表面分子;矿化诱导/成脂诱导后其可以分化为成骨细胞/成脂细胞;QPCR结果显示,用shRNA转染成功敲除了SCAPs中METTL3(P<0.05);CCK-8显示敲除SCAPs中的METTL3后,其增殖能力显著下降(P<0.05);划痕实验和跨孔实验均表明敲除SCAPs明显降低该细胞的迁移能力,差异均有统计学意义(P<0.05)。结论:METTL3在SCAPs的增殖及迁移能力中发挥促进作用。Objective To explore the influence of the METTL3 on proliferation and migration of murine SCAPs.Method Mouse SCAPs were isolated and cultured;We used the flow cytometry to identify the stem cell surface marker molecules.Alizarin red staining and Oil red O staining were used to identify the multiple differentiation potential of SCAPs.The METTL3 of SCAPs was knocked down by shRNA.Cell proliferation ability and migration capacity were checked by CCK-8 assay,wound healing test and transwell assay.changes in migration ability.Results The cells expressed stem cell surface markers highly which were found by Flow cytometry showed.After mineralization-inducing and adipogenic inducing,The cells counld differentiate into osteo/odontogenic and adipogenic cell.The METTL3 of SCAPs was successfully knocked down by shRNA transfection(P<0.05).CCK-8 showed that the METTL3knockdown in SCAPs reduced proliferative ability(P<0.05).The migratory ability of SCAPs was inhibited after knocking down METTL3 in SCAPs which were identified by wound healing test and transwell assay(P<0.05).Conclusion METTL3 plays a facilitating role in the proliferation and migration ability of SCAPs.

关 键 词：增殖 迁移 干细胞 

分 类 号：R3[医药卫生—基础医学]