氢化酶固定化研究进展  

Advance in the immobilization of hydrogenases

作　　者：雷航彬 何宁[1] 李斐煊 董玲玲 王世珍 LEI Hangbin;HE Ning;LI Feixuan;DONG Lingling;WANG Shizhen(Department of Chemical and Biochemical Engineering,College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005,Fujian,China;The Key Lab for Synthetic Biotechnology of Xiamen City,Xiamen University,Xiamen 361005,Fujian,China)

机构地区：[1]厦门大学化学化工学院化学工程与生物工程系,福建厦门361005 [2]厦门大学厦门市合成生物学重点实验室,福建厦门361005

出　　处：《合成生物学》2024年第6期1485-1497,共13页Synthetic Biology Journal

基　　金：国家重点研发计划“合成生物学”重点专项“糖水氢电系统——体外多酶高效产氢及氢电装置的基础及工程研究”(2022YFA0912003)。

摘　　要：氢化酶催化氢气向质子和电子的可逆转化,具有广阔的工业应用前景。但游离的氢化酶存在着对氧气敏感、传递电子速率慢等缺点。本文综述了碳材料、金属及半导体、高分子和金属-有机框架材料(MOF)固定化氢化酶。碳材料具有价格低廉、比表面积大等优势。金属及半导体有着良好的导电性能和优异的催化性能。高分子材料具有良好的生物相容性和机械性能,可以提高氢化酶的稳定性和对氧气的耐受性。MOF比表面积大,可设计调控,为理化性质不同的氢化酶提供了广泛的载体选择。复合材料固定化氢化酶可以结合不同材料的优势,拓宽固定化氢化酶的应用场景。固定化氢化酶可用于氢气的高效生产与应用以及生物不对称加氢制备手性化合物,为转变能源结构、实现绿色转型、解决环境问题提供了可选方案。Hydrogenases catalyze the reversible conversion of hydrogen gas into protons and electrons which is promising for industrial application.However,free hydrogenases face challenges such as oxygen sensitivity and low electron transfer rates.This review summarized the immobilization of hydrogenases by carbon materials,metals,semiconductors,polymers and metal-organic-frameworks(MOFs).Carbon materials provide the advantages of low cost and large specific surface areas,while they tend to agglomerate.Hydrogenases are immobilizated on carbon materials through adsorption,usually involving electrostatic interactions and hydrophobic interactions,and are used in bioelectrocatalysis,biofuel cells and bioreactors.Metals and semiconductors,known for high conductivity and excellent reactive activity,are expensive and less stable.Through adsorption involving electrostatic interaction and hydrophobic interaction,immobilization of hydrogenases on metals and semiconductors are normally applied in bioelectrocatalysis,biofuel cells and photoelectrocatalysis.Polymers have good biocompatibility and mechanical strength but low conductivity.Immobilization of hydrogenases on polymers can improve the stability and oxygen tolerance of hydrogenases.Immobilization on polymers is realized through adsorption and entrapment,involving hydrogen bonds,hydrophobic interactions andπ-πinteractions,and is often used in bioelectrocatalysis and photoelectrocatalysis.MOFs are designable and have high specific surface areas,which provide wide choices for hydrogenases immobilization.However,MOFs tend to collapse in harsh conditions.Immobilization on MOFs through adsorption and entrapment involves coordinate bonds,hydrophobic interaction,andπ-πinteraction.Furthermore,the prospect of immobilization of hydrogenases by novel hybrid materials was proposed which can expand the applications of immobilized hydrogenases.Immobilization of hydrogenases facilitates the stability of hydrogenases,which can be applied in efficient production and application of hydrogen,

关 键 词：氢化酶固定化 生物电催化 碳材料 半导体材料 高分子材料 金属-有机框架(MOF) 

分 类 号：Q814.2[生物学—生物工程]