过氧化物酶的重组表达和应用研究进展  

Research progress in recombinant expression and application of peroxidases

作　　者：李庚 申晓林[1] 孙新晓[1] 王佳[1] 袁其朋[1] LI Geng;SHEN Xiaolin;SUN Xinxiao;WANG Jia;YUAN Qipeng(State Key Laboratory of Chemical Resource Engineering,College of Life Science and Biotechnology,Beijing University of Chemical Technology,Beijing 100029,China)

机构地区：[1]北京化工大学生命科学与技术学院,化工资源有效利用国家重点实验室,北京100029

出　　处：《合成生物学》2024年第6期1498-1517,共20页Synthetic Biology Journal

基　　金：国家重点研发计划(2022YFC2106100);国家自然科学基金(22378016)。

摘　　要：过氧化物酶作为一类自然界中广泛分布的酶,参与了生物体的先天免疫防疫、植物微生物抗氧化应激、真菌木质素降解、植物细胞壁代谢及伤口愈合等重要生命过程。随着测序、基因编辑、重组蛋白表达和高通量筛选技术的飞速发展,越来越多的过氧化物酶被发现、表征和重组表达。这些种类丰富、数量庞大及催化性能卓越的过氧化物酶,在众多领域的应用研究中受到广泛关注。近年来过氧化物酶在重组表达上取得了显著进展,进一步促进了其在应用研究领域的开发。本文从系统进化分类及功能角度对过氧化物酶进行了简要概述,对近年来过氧化物酶在大肠杆菌、酵母和丝状真菌中重组表达研究进展及其在环境修复、化合物检测的应用研究成果进行系统综述,重点介绍了过氧化物酶应用于生物合成高附加值化合物方面的最新研究进展,并对其目前在该领域应用研究中存在的底物和产物非专一性问题及辅因子H2O2细胞毒性问题进行讨论。过氧化物酶在医学检测、环境保护和生物合成等领域中的应用潜力巨大。然而,当前的技术和应用仍面临一些挑战,比如过氧化物酶在复杂环境中的稳定性和活性差、酶制剂生产成本高及专一性差问题。未来,通过结合蛋白质工程、合成生物学和固定化技术等多学科的最新进展,可以有效解决这些挑战,推动过氧化物酶在各个领域的广泛应用。As a class of enzymes widely distributed in nature,peroxidases are involved in important life processes such as innate immunity and epidemic prevention of organisms,anti-oxidative stress of plant microorganisms,fungal lignin degradation,plant cell wall metabolism and wound healing.With the rapid development of DNA sequencing,gene editing,recombinant protein expression and high-throughput screening technologies,more and more peroxidases have been discovered,characterized and recombinantly expressed.These peroxidases,characterized by their species diversity,abundant quantity,and excellent catalytic performance,have attracted extensive attention in many fields of application research.In recent years,remarkable progress has been made in the recombinant expression of peroxidases,further promoting their development in the field of applied research.Additionally,as we deepen our understanding of the catalytic properties of peroxidases,new opportunities have emerged for their application in the field of biosynthesis.Their high catalytic activity allows for rapid oxidation reactions under mild conditions and enables the construction of multi-enzyme cascade systems in conjunction with other enzymes,thereby facilitating the efficient synthesis of complex compounds.This paper provides a brief overview of peroxidases from the perspective of systematic evolutionary classification and function.It systematically reviews recent progress in the recombinant expression of peroxidases in Escherichia coli,yeast,and fungi,as well as their application achievements in environmental remediation and compound detection.The focus is on the latest research advances in the application of peroxidases for the biosynthesis of high-value-added compounds.The paper also discusses the current issues in this field,such as substrate and product non-specificity and the cytotoxicity of the cofactor H2O2.Peroxidases have enormous potential for applications in medical diagnostics,environmental protection,and biosynthesis.However,current technologies and app

关 键 词：过氧化物酶 蛋白重组表达 污染物降解 化合物检测 生物合成 

分 类 号：Q812[生物学—生物工程]