向天果提取物对阿霉素所致心肌细胞损伤的作用及机制  

Effect of Swietenia macrophylla extracts on adriamycin-induced myocardial cell injury and its mechanisms

作　　者：叶容芳 熊绍风 徐洁 黄江龙[3,4] YE Rongfang;XIONG Shaofeng;XU Jie;HUANG Jianglong(Department of Pharmacy,the First Affiliated Hospital,Jiangxi Medical College,Nanchang University,Nanchang 330006,Jiangxi Province,China;Department of Emergency,the First Affiliated Hospital,Jiangxi Medical College,Nanchang University,Nanchang 330006,Jiangxi Province,China;Department of Radiology,the Second Affiliated Hospital,Jiangxi Medical College,Nanchang University,Nanchang 330006,Jiangxi Province,China;Jiangxi Provincial Key Laboratory of Intelligent Medical Imaging,Nanchang 330006,Jiangxi Province,China)

机构地区：[1]南昌大学第一附属医院药学部,江西南昌330006 [2]南昌大学第一附属医院急诊科,江西南昌330006 [3]南昌大学第二附属医院医学影像中心,江西南昌330006 [4]智能医学影像江西省重点实验室,江西南昌330006

出　　处：《新乡医学院学报》2025年第1期15-24,共10页Journal of Xinxiang Medical University

基　　金：江西省卫生健康委科技计划一般项目(编号:202310457)。

摘　　要：目的探讨向天果提取物对阿霉素所致心肌细胞损伤的作用及其机制。方法将对数生长期H9C2细胞随机分为对照组、1 g·L^(-1)向天果提取物组、10 g·L^(-1)向天果提取物组、50 g·L^(-1)向天果提取物组、100 g·L^(-1)向天果提取物组、200 g·L^(-1)向天果提取物组和400 g·L^(-1)向天果提取物组。对照组细胞加入不含药物的完全培养基,1 g·L^(-1)向天果提取物组、10 g·L^(-1)向天果提取物组、50 g·L^(-1)向天果提取物组、100 g·L^(-1)向天果提取物组、200 g·L^(-1)向天果提取物组和400 g·L^(-1)向天果提取物组细胞分别加入1、10、50、100、200、400 g·L^(-1)向天果提取物10 mL干预24 h。采用细胞计数试剂盒-8(CCK-8)法检测各组H9C2细胞存活率,乳酸脱氢酶(LDH)试剂盒检测各组H9C2细胞中LDH水平。另取对数生长期H9C2细胞随机分为对照组、阿霉素组、阿霉素+铁死亡抑制剂ferrostabin-1(Fer-1)组、阿霉素+向天果提取物组,对照组细胞加入不含药物的完全培养基,阿霉素组细胞加入1μmol·L^(-1)阿霉素10 mL,阿霉素+Fer-1组细胞加入1μmol·L^(-1)阿霉素和2μmol·L^(-1) Fer-1各10 mL,阿霉素+向天果提取物组细胞加入100 g·L^(-1)向天果提取物10 mL干预24 h后再加入1μmol·L^(-1)阿霉素10 mL处理48 h;采用CCK-8法检测各组H9C2细胞存活率,LDH检测试剂盒检测各组H9C2细胞中LDH水平,流式细胞仪检测各组H9C2细胞凋亡率,酶联免疫吸附法检测各组H9C2细胞中caspase-3水平,铁含量试剂盒检测H9C2细胞中铁离子含量,Western blotting法检测各组细胞中前列腺素内过氧化物合酶2(PTGS2)、谷胱甘肽过氧化物酶4(GPX4)、p62、人自噬相关基因Beclin-1蛋白表达水平,单丹磺酰尸胺法检测各组H9C2细胞自噬体荧光强度。结果对照组、1 g·L^(-1)向天果提取物组、10 g·L^(-1)向天果提取物组、50 g·L^(-1)向天果提取物组、100 g·L^(-1)向天果提取物组细胞存活率及细胞中LDH水平�Objective To investigate the effect of Swietenia macrophylla extracts on adriamycin-induced myocardial cell injury and its mechanisms.Methods H9C2 cells in the logarithmic growth phase were randomly divided into a control group and 1,10,50,100,200,and 400 g·L^(-1) Swietenia macrophylla extract groups.Cells in the control group were placed in a complete medium without adding any drug,while cells in the other groups were treated separately with 10 mL of 1,10,50,100,200,and 400 g·L^(-1) Swietenia macrophylla extracts for 24 hours.Then,cell viability was assessed by the cell counting kit-8(CCK-8)method,and lactate dehydrogenase(LDH)levels in H9C2 cells were measured by an LDH assay kit.Additionally,H9C2 cells in the logarithmic growth phase were randomly divided into a control group,a adriamycin group,a adriamycin+ferrostabin-1(Fer-1)group,and a adriamycin+Swietenia macrophylla extract group.Cells in the control group were cultured in a complete medium containing no drug.Cells in the adriamycin group were treated with 10 mL of 1μmol·L^(-1) adriamycin,and those in the adriamycin+Fer-1 group were treated with 10 mL of 1μmol·L^(-1) adriamycin and 10 mL of 2μmol·L^(-1) Fer-1.Cells in the adriamycin+Swietenia macrophylla extract group were treated first with 10 mL of 100 g·L^(-1) Swietenia macrophylla extracts for 24 hours and then with 1μmol·L^(-1) adriamycin for 48 hours.Cell viability of each group was assessed by the CCK-8 method,and LDH levels were measured by an LDH assay kit.Apoptosis rates were detected by flow cytometry,and caspase-3 levels in H9C2 cells were measured by enzyme-linked immunosorbent assay.Ferri ion contents were detected by ferri iron content assay kit.The expression levels of prostaglandin-endoperoxide synthase 2(PTGS2),glutathione peroxidase 4(GPX4),p62,and human autophagy-related gene Beclin-1 proteins were detected by Western blotting.Autophagosome fluorescence intensity of H9C2 cells was measured using monodansylcadaverine staining.Results There was no statistically significant dif

关 键 词：向天果提取物 阿霉素 心肌细胞损伤 自噬 铁死亡 

分 类 号：R541[医药卫生—心血管疾病]