基于Zm00001d025794基因的玉米子粒脱水性状关联分析  

Association Analysis of Maize Grain Dehydration Related Traits Based on Zm00001d025794 Gene

作　　者：李佳雨 张子然 李冉冉 渠建洲 马丽[1] 薛吉全[1] 徐淑兔[1] LI Jia-yu;ZHANG Zi-ran;LI Ran-ran;QU Jian-zhou;MA Li;XUE Ji-quan;XU Shu-tu(College of Agronomy,Northwest A&F University/Key Laboratory of Maize Biology and Genetic Breeding in Arid Area of Northwest Region,Yangling 712100,China)

机构地区：[1]西北农林科技大学农学院/西北旱区玉米生物学与遗传育种重点实验室,陕西杨凌712100

出　　处：《玉米科学》2024年第9期11-19,共9页Journal of Maize Sciences

基　　金：陕西省农业协同创新与推广联盟示范推广项目(LM2021-02);杨凌种业创新中心重点研发专项(YLZY-ym-01)。

摘　　要：随着全程机械化技术的发展,玉米成熟后期子粒脱水速率成为影响玉米生产的重要因素。有必要开展子粒脱水速率的遗传解析,挖掘可用于分子标记辅助选择育种的有利等位基因。候选基因的关联分析是目前有利等位基因挖掘的有效方法之一。使用91份玉米自交系对前期全基因组关联分析筛选获得的子粒脱水相关基因Zm00001d025794进行候选基因关联分析,找到该基因16个与子粒脱水速率显著相关的多态性位点。进一步结合不同脱水速率材料中的子粒发育不同时期的转录组数据,发现该基因在子粒发育后期表达量逐渐下降,且在脱水快的材料中表达量下降更快。最后选取3个与42DAP-63DAP的子粒脱水速率(KDR6342)显著关联的位点(P<0.05)进行单倍型分析,获得了最优单倍型。With the advancement of full-process mechanization technology,the grain dehydration rate during the late maturity stage has become an important factor affecting maize production.It is necessary to carry out genetic analysis of grain dehydration rate,and to explore the favorable and usable alleles for molecular marker assisted selection breeding.Candidate gene association analysis is one of the effective methods for mining favorable alleles.In this study,91 maize inbred lines were used to conduct candidate gene association analysis for the target gene Zm00001d025794,which were associated with grain dehydration rate by genome wide association analysis.Sixteen polymorphic sites significantly related to grain dehydration rate.Further combining the transcriptome data at different stages of grain development in materials with different dehydration rates,it was found that the expression of this gene decreased gradually at the late stage of grain development and decreased faster with fast dehydration.Finally,three loci significantly(P<0.05) associated with grain dehydration rate(KDR6342) during 42DAP-63DAP's period were selected for haplotype analysis,and the optimal haplotype was obtained.

关 键 词：玉米 子粒脱水速率 候选基因关联分析 基因表达 

分 类 号：S513.035.3[农业科学—作物学]