副猪格拉瑟菌血清8型弱毒菌株生物学特性分析  

Biological characteristics of medium virulent strain of Glaesserella parasuis identified as serovar 8

作　　者：王治方[1] 徐引弟[1] 朱文豪[1] 焦文强[1] 李海利[1] 张立宪[1] 游一[1] 张家庆[1] 蔡旭旺[2] 徐晓娟[2] 白红杰[1] 许峰[1] 雷亚楠 郭紫林[1] WANG Zhifang;XU Yindi;ZHU Wenhao;JIAO Wenqiang;LI Haili;ZHANG Lixian;YOU Yi;ZHANG Jiaqing;CAI Xuwang;XU Xiaojuan;BAI Hongjie;XU Feng;LEI Ya'nan;GUO Zilin(Institute of Animal Husbandry Research,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;State Key Laboratory of Agricultural Microbiology,College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]河南省农业科学院畜牧研究所,河南郑州450002 [2]华中农业大学动物医学院,农业微生物国家重点实验室,湖北武汉430070

出　　处：《中国兽医科学》2024年第12期1627-1634,共8页Chinese Veterinary Science

基　　金：国家重点研发计划项目(2021YFD1301205);河南省农业科学院自主创新项目(2023ZC055)。

摘　　要：为了掌握副猪格拉瑟菌临床弱毒菌株的病原特征,试验对猪格拉瑟氏病临床疑似病例无菌采集肺、气管、心包液等样品,通过细菌分离纯化、镜检、生化鉴定、基因扩增测序、豚鼠致病性试验及药敏试验等方法,对分离菌株进行鉴定分型和病原特性研究分析。证实从肺样品中分离获得病原菌为副猪格拉瑟菌血清8型菌株,该菌株携带vta1、vta2、vta3等多个毒力基因,对豚鼠有一定的致病性,但毒力较弱。该菌株携带有氨基糖苷类、四环素类、磺胺类药物多个耐药基因;该菌耐药表型有明显的多重耐药性,对复方磺胺甲恶唑、氨基糖苷类和四环素类表现出明显抗性,耐药表型和耐药基因型基本吻合。该菌对头孢类、氟苯尼考、大环内酯类和喹诺酮类药物表现敏感,临床用药防治效果显著。该研究结果为副猪格拉瑟菌血清8型流行病学调查及临床防控提供了参考资料。In order to illustrate the pathogenic characteristics of the clinically weakly virulent strains of Glaesserella parasuis(Gps).The lung,trachea,pericardial fluid and other samples were collected aseptically from clinical suspected cases of Gr?sser's disease in pigs,The isolated strains were identified and analyzed for their pathogenic characteristics through bacterial isolation and purification,microscopic examination,biochemical identification,16S rRNA amplification and sequencing,guinea pig pathogenicity testing,and drug sensitivity testing of agar diffusion.It was confirmed that the pathogenic bacterium isolated from the lung samples was the serotype 8 of Gps,which carried multiple virulence genes such as vta1,vta2,vta3,and had a certain degree of pathogenicity to guinea pigs,but the virulence was relatively weak.And the strain carried multiple resistance genes to aminoglycosides,tetracyclines,and sulphonamides,and clinically showed obvious multi-drug resistance,and shows significant resistance to compound sulfamethoxazole,aminoglycosides,and tetracyclines,and the resistance phenotypes and drug-resistant genotypes were basically matched.The isolated Gps in the current research was susceptible to cephalosporins,florfenicol,macrolides and quinolones,and the effect of clinical application were significant.The results of this study provide reference information for the epidemiological investigation and clinical prevention and control of serotype 8 of Gps.

关 键 词：格拉瑟菌血清8型 毒力基因 致病性试验 药敏试验 耐药基因 

分 类 号：S852.612[农业科学—基础兽医学]