闭合性颅脑损伤模型中GFAP和VEGF的表达研究  

Expression study of GFAP and VEGF in a closed craniocerebral injury model

作　　者：张曙光 党志强[3] 巩海霞 刘长海 贾富全 Zhang Shuguang;Dang Zhiqiang;Gong Haixia;Liu Changhai;Jia Fuquan(Department of Forensic Medicine,Inner Mongolia Medical University,Hohhot 010059;Key Laboratory of Molecular Pathology of Inner Mongolia Autonomous Region,Hohhot 010059;Inner Mongolia Dean Judicial Appraisal Center,Hohhot 010010;Ulanqab people's procuratorate,Ulanqab 012001)

机构地区：[1]内蒙古医科大学基础医学院法医学教研室,内蒙古呼和浩特010059 [2]内蒙古医科大学内蒙古自治区分子病理学重点实验室,内蒙古呼和浩特010059 [3]内蒙古迪安司法鉴定中心,内蒙古呼和浩特010010 [4]乌兰察布市人民检察院,内蒙古乌兰察布012001

出　　处：《中国法医学杂志》2024年第6期685-689,704,共6页Chinese Journal of Forensic Medicine

基　　金：内蒙古自治区自然科学基金项目(2023LHMS08027);内蒙古医科大学基础医学院育才计划重点实验室开放基金项目(NYFZBL202206);内蒙古医科大学“创客培育”项目(101322024015)。

摘　　要：目的探讨大鼠闭合性脑损伤后GFAP和VEGF的表达规律,并以此推断脑损伤后经过时间,进而为损伤时间推断提供参考。方法选取雄性大鼠随机分为对照组和实验组,其中实验组按照损伤后经过时间不同又分损伤后1 h、6 h、12 h、1 d、3 d、7 d、14 d组。采用免疫组化法和ELISA法检测大鼠闭合性脑损伤后脑组织及血清中GFAP和VEGF的表达情况。结果(1)免疫组化各实验组中,GFAP主要在星形胶质细胞胞浆中表达,伤后1 h开始明显增多、7 d内表达进行性增高,到伤后14 d时表达下降接近对照组水平;VEGF主要在神经元胞浆中表达,伤后1 d时明显增多,到伤后3 d时达高峰、14 d时表达减少;(2)ELISA各实验组血清中VEGF含量升高早于脑组织中VEGF表达,伤后6 h开始明显增多,到伤后3 d时含量最多、伤后14 d时接近对照组。结论(1)GFAP与VEGF表达呈现出时序性变化的特点,两者有望成为法医推断损伤时间的生物学标记物;(2)GFAP和VEGF有较高敏感性和特异性,对早期颅脑损伤时间推断有重要意义。Objective To investigate the expression pattern of GFAP and VEGF after closed brain injury in rats,and to infer the time elapsed after brain injury,and then to provide reference for the inference of injury time.Methods Male rats were randomly divided into control group and experimental group,in which the experimental group was divided into 1 h,6 h,12 h,1 d,3 d,7 d and 14 d groups according to the different elapsed time after injury.Immunohistochemistry and ELISA were used to detect the expression of GFAP and VEGF in brain tissue and serum of rats after closed brain injury.Results(1)In each immunohistochemistry experimental group,GFAP was mainly expressed in the cytoplasm of astrocytes,with an obvious increase at 1 h after injury,a progressive increase of expression in 7 d,and a decrease of expression close to the level of the control group at 14 d after injury.While VEGF was mainly expressed in the cytoplasm of neurons,with an obvious increase at 1 d after injury,a peak at 3 d post-injury,and a decrease in expression at 14 d.(2)In each ELISA experimental group,the VEGF content in serum of each experimental group increased earlier than the VEGF expression in brain tissue,which began to increase significantly at 6 h after injury,and the content was the most at 3 d after injury,and was close to that of the control group at 14 d after injury.Conclusion(1)It has showed temporal changes in the expression of GFAP and VEGF,and both are expected to be biological markers for inferring the time of injury in forensic work.(2)GFAP and VEGF have high sensitivity and specificity and are important for the extrapolation of early craniocerebral injury time.

关 键 词：法医病理学 闭合性脑损伤 GFAP VEGF 

分 类 号：D919.1[医药卫生—法医学]