小檗碱对金黄色葡萄球菌差异基因表达的调控作用及其机制  

Effect of berberine on regulation of differential gene expression in Staphylococcus aureus

作　　者：周芳芳 谷雪妹 林明[1] 王蕾[1] ZHOU Fang-fang;GU Xue-mei;LIN Ming;WANG Lei(Shanghai Eighth People's Hospital,Shanghai 200235,China)

机构地区：[1]上海市第八人民医院检验科,上海200235

出　　处：《中华医院感染学杂志》2024年第23期3526-3532,共7页Chinese Journal of Nosocomiology

基　　金：上海市卫生健康委员会基金资助项目(ZHYY-ZXYJHZX-202104);上海市徐汇区医学重点学科基金资助项目(SHXHZDXK202322)。

摘　　要：目的分析小檗碱作用后金黄色葡萄球菌[甲氧西林敏感金黄色葡萄球菌(MSSA)和甲氧西林耐药金黄色葡萄球菌(MRSA)]的全基因组序列表达特征的差异,分析MSSA、MRSA菌株基因及调控通路的差异性。方法高浓度[1/2最低抑菌浓度(MIC),64μg/ml]、低浓度(1/8 MIC,16μg/ml)及未加小檗碱分别处理标准菌株ATCC25923、USA 3003 h,通过Illumina Hiseq平台进行测序,运用DEseq2和PossionDis算法进行差异表达基因(DEGs)检测。分析ATCC 25923、USA 300差异基因的表达情况;将差异基因筛选条件设为P<0.05,且差异倍数>2。结果通过RNA-seq测序发现,ATCC 25923和USA 300菌株染色体全基因组序列为2863023518 bp、4617448903 bp,基因组数量为2154、2304个,编码基因GC含量为35.34%、37.09%,平均基因大小为147、144 bp;高浓度组与正常对照组相比,MRSA共有859个差异基因表达,而MSSA仅有276个差异基因表达;低浓度组与正常对照组相比,MRSA共有19个差异基因表达,而MSSA有39个差异基因表达;小檗碱对MSSA调控的GO通路有显著差异的通路共有148条(P<0.05);对MRSA调控的GO通路有显著差异的通路296条(P<0.05),其中共同的调节通路有48条通路;小檗碱对MSSA调控KEGG通路涉及71条通路,而对MRSA调控的KEGG通路涉及104条通路,其中差异调节通路有43条。结论两株菌株基因组序列大小差异比较明显,但基因组数量、编码基因GC含量及平均基因大小无明显差异。研究小檗碱作用后ATCC 25923、USA 300转录组差异基因表达情况,推测小檗碱主要影响膜多糖生物合成蛋白质家族、毒力基因、GO通路及KEGG通路发挥对MSSA、MRSA的抑菌作用。OBJECTIVE To analyze the differences in genome-wide sequence expression characteristics of Staphylococcus aureus strains(methicillin-sensitive and methicillin-resistant)following berberine treatment,and to analyze the disparities in the genes and regulatory pathways of MSSA and MRSA strains.METHODS The standard strains ATCC 25923 and USA300 were treated with high concentration[1/2 minimum inhibitory concentration(MIC),64μg/ml],low concentration(1/8 MIC,16μg/ml),and no berberine for three hours,the standard strains were sequenced using the Illumina Hiseq platform,and the differentially expressed genes(DEGs)was detected by applying DEseq2 and PosionDis algorithms.The expression of ATCC 25923 and USA 300 differential genes was analyzed,and the differential gene screening conditions were set to P value<0.05 and multiplicity of difference>2.RESULTS Subsequent RNA-seq sequencing revealed that the whole genome sequences of the chromosomes of ATCC 25923 and USA300 strains were 28,630,235bp and 46,174,489 bp,respectively,with 2,154 and 2,304 genomes,and the GC content of the coding genes were found to be 35.34%and 37.09%,and the average gene sizes were found to be 147 bp and 144 bp,respectively.In the high concentration group compared with the normal control group,MRSA had a total of 859 differential gene expressions,while MSSA had only 276 differential gene expression.In the low concentration group compared with the normal control group,MRSA had a total of 19 differential gene expressions,while MSSA had 39 differential gene expressions.There were 148 GO pathways with significant differences of pathways regulated by berberine on MSSA(P<0.05),296 GO pathways with significant differences of pathways regulated by berberine on MRSA(P<0.05),of which 48 pathways were co-regulated.Berberine was associated with 71 KEGG path ways for regulating MSSA and 104 KEGG pathways for regulating MRSA,with 43 differentially regulated pathways.CONCLUSIONS The differences in genome sequence size between the two strains were relatively obviou

关 键 词：小檗碱 差异基因 作用机制 

分 类 号：R378[医药卫生—病原生物学]