牙种植修复患者miR-34a和Wnt/β-catenin信号通路对术后感染的预测价值  

Predictive value of miR-34a and Wnt/β-catenin signaling pathway for postoperative infection in patients with dental implant restorations

作　　者：舒献碧 杨庆 龙秋静 SHU Xian-bi;YANG Qing;LONG Qiu-jing(Deyang Stomatological Hospital,Deyang,Sichuan 618000,China)

机构地区：[1]德阳市口腔医院种植修复科,四川德阳618000

出　　处：《中华医院感染学杂志》2024年第24期3774-3778,共5页Chinese Journal of Nosocomiology

基　　金：四川省医学科研基金资助项目(S21023)。

摘　　要：目的探讨牙种植修复患者术后感染微小RNA-34a(miR-34a)、Wnt/β-catenin信号通路,并分析其对术后感染的预测价值。方法选取2021年10月-2023年9月于德阳市口腔医院口腔科接受牙种植体修复术患者211例纳入研究,根据术后是否发生感染纳入感染组(n=60)、非感染组(n=151)。比较两组患者miR-34a、Wnt mRNA、β-catenin mRNA及白细胞介素-6(IL-6)、IL-1β、肿瘤坏死因子-α(TNF-α)水平。采用Pearson相关性分析法分析miR-34a与Wnt mRNA、β-catenin mRNA及IL-6、IL-1β、TNF-α的相关性。采用受试者工作特征(ROC)曲线分析miR-34a、Wnt mRNA、β-catenin mRNA对牙种植修复患者术后感染的预测价值。结果60例术后感染患者共培养分离病原菌68株,其中厌氧菌54株(79.41%),需氧菌14株(20.59%);感染组miR-34a为(1.58±0.46)高于非感染组(0.98±0.35),Wnt mRNA、β-catenin mRNA为(1.97±0.58)、(3.59±1.21)均低于非感染组(2.83±0.82)、(4.67±1.69)(P<0.05);miR-34a、Wnt mRNA、β-catenin mRNA单独及联合检测曲线下面积(AUC)分别为0.812、0.756、0.776与0.891,联合检测的预测效能优于单独检测。结论miR-34a、Wnt/β-catenin信号通路参与牙种植体术后感染的发生与发展,其机制可能与miR-34a靶向抑制Wnt/β-catenin信号通路促进感染的发生。OBJECTIVE To investigate the predictive value of miR-34a,Wnt/β-catenin signaling pathways for post-operative infection in patients with dental implant restorations and analyze the values of the indexes in prediction of the postoperative infection.METHODS A total of 211 patients who received dental implant restorations in depart-ment of stomatology,Deyang Stomatological Hospital from Oct.2021 to Sep.2023 were included in the study,and they were included in the infected group(n=6o)and the non-infected group(n=151)according to whether the postoperative infection occurred.The levels of miR-34a,Wnt mRNA,β-catenin mRNA,interleukin-6(IL-6),IL-1p,and tumor necrosis factorα(TNF-α)were compared between the two groups.The correlation between miR-34a and the levels of Wnt mRNA,β-catenin mRNA,IL-6,IL-1βand TNF-αwere analyzed by Pearson corre-lation analysis.Receiver operating characteristic(ROC)curve was used to analyze the predictive value of miR-34a,Wnt mRNA andβ-catenin mRNA alone and in combination for postoperative infection in patients with dental im-plant restorations.RESULTS A total of 68 strains of pathogenic bacteria were co-cultured in 60 patients with post-operative infection,of which 54 were anaerobes(79.41%)and 14 were aerobes(20.59%).The miR-34a in the in-fected group was(1.58±0.46),higher than that in the non-infected group(0.98±0.35),while the levels of Wnt mRNA andβ-catenin mRNA were(1.97±0.58)and(3.59±1.21),lower than those of non-infected group(2.83±0.82)and(4.67±1.69)(P<0.05).The area under the curve(AUC)of miR-34a,Wnt mRNA andβ-cate-nin mRNA alone and in combination were 0.812,0.756,0.776 and 0.891,respectively,and the predictive efficacy of the combined assay was better than that of single detection.CONCLUSION The miR-34a and Wnt/β-catenin sig-naling pathway was involved in the occurrence and development of postoperative dental implant infections,and the mechanism might be related to the targeted inhibition of the Wnt/β-catenin signaling pathway by miR-34a to pro-mote infection.

关 键 词：牙种植修复术 种植体周围感染 预测价值 微小RNA-34a WNT/Β-CATENIN信号通路 白细胞介素-6 肿瘤坏死因子-α 

分 类 号：R619.3[医药卫生—外科学]