参葛补肾胶囊及其多糖黄酮对幼龄小鼠性早熟的影响  

Effect of Shenge Bushen Capsules and Its Polysaccharides and Flavonoids on Precocious Puberty in Young Mice

作　　者：孙虹 雷帆[2] 李成功 胡时先 王卫华[4] 任宾 郝娟 罗睿 杜力军[2] SUN Hong;LEI Fan;LI Chenggong;HU Shixian;WANG Weihua;REN Bin;HAO Juan;LUO Rui;DU Lijun(Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100094,China;School of Life Sciences,Tsinghua University,Beijing 100084,China;Center of Pharmacy R&D,XinJiang Huachun Bio-Pharmaceutical Co.Ltd.,Urumqi 830000,China;Pharmacy Technology Center,Tsinghua University,Beijing 100084,China)

机构地区：[1]中国医学科学院中国协和医学院药用植物研究所,北京100094 [2]清华大学生命科学学院,北京100084 [3]新疆华春生物药业有限责任公司药业研发中心,乌鲁木齐830000 [4]清华大学药学技术中心,北京100084

出　　处：《中国实验方剂学杂志》2025年第1期95-103,共9页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家“重大新药创制”科技重大专项(2018ZX09731020)。

摘　　要：目的:探讨参葛补肾胶囊(简称参葛补肾)对正常3周龄小鼠性发育的作用。方法:实验共分2部分:第1部分将小鼠分为4组,正常组及参葛补肾低、中、高剂量组(给药剂量分别为234.7、469.4、938.7 mg·kg^(-1))。第2部分小鼠共分4组,分别为正常组,太子参多糖组、总黄酮组、参葛补肾组,给药剂量均为469.4 mg·kg^(-1)。给药7 d观察雌性小鼠的阴道开口及雄性小鼠的睾丸下降阴囊数及其卵巢和睾丸脏器系数等,给药4周后雌雄合笼2 d,观察雌鼠受孕率,孕鼠生产后继续给药母鼠4周,观察子鼠阴道开口数睾丸下降阴囊数及其卵巢和睾丸脏器系数等。采用酶联免疫吸附测定法(ELISA)检测血清性激素,采用蛋白免疫印迹法(Western blot)检测下丘脑促性腺激素释放激素蛋白(GnRH)和生长激素蛋白(GH)的表达。结果:与正常组比较,参葛补肾给药7 d,对各组雌性小鼠的阴道开口及雄性小鼠的睾丸下降影响差异无统计学意义,给药4周参葛补肾低剂量组雌鼠的受孕率明显下降(P<0.05),其他影响差异无统计学意义。3剂量对卵巢睾丸脏器系数均影响无统计学意义,对下丘脑GnRH及GH表达作用差异无统计学意义,参葛补肾的主要组成太子参多糖给药7 d雌性小鼠阴道开口数明显减少(P<0.05),给药4周时未孕雌鼠血清雌二醇水平明显下降(P<0.05),但对雌雄鼠下丘脑GnRH、GH蛋白表达影响均差异无统计学意义;对子代小鼠在阴道开口和睾丸下降等性早熟指标均无明显影响。结论:参葛补肾对幼年小鼠性早熟无明显促进作用,对成年鼠受孕率及其子鼠性发育无明显影响。Objective:To explore the effect of Shenge Bushen Capsules(SBC)on sexual development in normal 3-week-old mice.Methods:The experiment consisted of two parts.In the first part,mice were divided into four groups:The control group and the low,medium,and high-dose SBC groups(234.7,469.4,938.7 mg·kg^(-1),respectively).In the second part,mice were divided into four groups:Control group,Pseudostellariae Radix polysaccharide(PRP)group,total flavonoids group,and SBC group,all receiving a dose of 469.4 mg·kg^(-1).After 7 days of administration,the vaginal opening of female mice and the descent of testes and scrotum in male mice,as well as the ovarian and testicular organ indices,were observed.After 4 weeks of administration,female and male mice were housed together for 2 days,and the pregnancy rate of females was monitored.After delivery,the pregnant female mice continued receiving the treatment for 4 weeks,and the sexual development of their offspring,including vaginal opening,testicular descent,and organ indices of ovaries and testes,was observed.Serum sex hormones were measured by enzyme-linked immunosorbent assay(ELISA),and the expression of gonadotropin-releasing hormone(GnRH)and growth hormone(GH)proteins in the hypothalamus was assessed by Western blot.Results:Compared with the control group,there was no significant effect on the vaginal opening of female mice or the descent of testes in male mice after 7 days of SBC administration.After 4 weeks of administration,the pregnancy rate in the low-dose group was significantly reduced(P<0.05),but no significant effects were observed in the other groups.The three doses of SBC did not significantly affect the ovarian or testicular organ indices,and there was no significant upregulation in the expression of GnRH or GH in the hypothalamus.The primary component of SBC,Pseudostellariae Radix polysaccharide,significantly reduced the vaginal opening in female mice after 7 days of administration(P<0.05).After 4 weeks,the serum estradiol levels of non-pregnant female mice were dec

关 键 词：参葛补肾胶囊 太子参多糖 总黄酮 性早熟 幼龄小鼠 雌二醇 睾酮 

分 类 号：R285[医药卫生—中药学] R289[医药卫生—中医学] R277