江苏部分地区鸡源大肠杆菌的分离鉴定及生物学特性研究  

Isolation,Identification and Biological Characteristics of Escherichia coli from Chicken in Some Areas of Jiangsu Province

作　　者：张萍 王静悦 刘海侠[2] 徐孝宙[2] 陈栋 薛媚[1] 蔡璨宇 方光远[1] ZHANG Ping;WANG Jingyue;LIU Haixia;XU Xiaozhou;CHEN Dong;XUE Mei;CAI Canyu;FANG Guangyuan(Jinling Institute of Technology,College of Animal Science and Food Engineering,Nanjing,Jiangsu 211169;Jiangsu Polytechnic College of Agricultural and Forestry,Zhenjiang,Jiangsu 212400)

机构地区：[1]金陵科技学院动物科学与食品工程学院,江苏南京211169 [2]江苏农林职业技术学院,江苏镇江212400

出　　处：《中国家禽》2025年第1期119-126,共8页China Poultry

基　　金：江苏省大学生创新训练计划项目(202313573101Y)。

摘　　要：为研究江苏地区鸡源大肠杆菌的血清型、毒力基因分布、进化分群及耐药性等生物学特性,试验从江苏部分地区养鸡场的疑似大肠杆菌感染病例中,分离并鉴定出鸡大肠杆菌44株,采用平板凝集法检测分离菌O抗原血清型,PCR法检测分离菌的毒力基因分布、系统进化分类,Kirby-Bauer纸片扩散法检测分离菌的耐药情况。结果显示:44株分离菌中O抗原血清型菌株有38株,共有17种血清型,其中O138(15.8%)、O86(13.2%)、O54(10.5%)和O14(10.5%)为优势血清型;fimC、yijp检出率最高,均为100%(44/44)携带,其次为mat、iucD、ompA、iss,分别为97.73%(43/44)、84.09%(37/44)、79.55%(35/44)、59.09%(26/44),且分离菌全部同时含有3种或以上的毒力基因;分离菌主要属于A、D群,占比分别为38.64%和31.83%,少数为B1、B2群,占比分别为11.36%和18.18%;44株分离菌均对红霉素、克林霉素、万古霉素100%耐药,对四环素、复方新诺明的耐药率均为86.36%,且多重耐药现象明显。研究提示,江苏部分地区鸡源大肠杆菌血清型复杂多样,携带多种毒力基因,且耐药问题比较严重,应加强当地耐药菌的监测,从而选择合适的药物防治鸡大肠杆菌病。To investigate the serotypes,virulence genes distribution,evolutionary clusters and drug resistance of Escherichia coli(E.coli)from chickens in Jiangsu province,44 strains of chicken E.coli were isolated and identified from suspected cases of E.coli infection in chicken farms in some areas of Jiangsu province.The plate agglutination method was used to detect the O-antigen serotypes of the isolates,PCR method was used to detect the distribution of virulence genes and the phylogenetic classification of isolates,and the Kirby-Bauer paper diffusion method was used to detect the drug resistance of isolates.The results showed that there were 38 O-antigen serotype strains of 44 isolates with a total of 17 serotypes,of which O138(15.8%),O86(13.2%),O54(10.5%)and O14(10.5%)were the dominant serotypes;fimC and yijp had the highest detection rate,which were 100%(44/44),followed by mat,iucD,ompA,and iss,with 97.73%(43/44),84.09%(37/44),79.55%(35/44),and 59.09%(26/44),respectively,and all the isolated strains contained simultaneously three or more virulence genes;The isolats mainly belonged to groups A and D,accounting for 38.64%and 31.83%,respectively,and a few belonged to groups B1 and B2,accounting for 11.36%and 18.18%,respectively.All 44 isolates were 100%resistant to erythromycin,clindamycin and vancomycin,86.36%resistant to tetracycline and cotrimoxazole,and the phenomenon of multiple drug resistance was obvious.The study suggested that the serotypes of E.coli from chickens in some areas of Jiangsu province were complex and diverse,carried multiple virulence genes,and had a serious drug resistance problem.It was necessary to strengthen the monitoring of local drug-resistant bacteria so as to select appropriate drugs to prevent and control of chicken colibacillosis.

关 键 词：鸡 大肠杆菌 毒力基因 耐药性 江苏 

分 类 号：S852.61[农业科学—基础兽医学]