肝癌锌代谢相关基因及SLC30A5序列特征分析  

Characterisation of Zinc Metabolism Related Genes and SLC30A5 Sequence in Hepatocellular Carcinoma

作　　者：程子鉴 Cheng Zijian(Southwest University)

机构地区：[1]西南大学

出　　处：《哈尔滨师范大学自然科学学报》2024年第4期87-94,共8页Natural Science Journal of Harbin Normal University

摘　　要：通过生物信息学技术分析锌代谢相关基因在肝癌中的表达特征,溶质运载家族30成员5(solutecarrierfamily30member5,SLC30A5)的序列特征.从GEO数据库下载肝癌、胃癌、胰腺癌和正常肝脏组织转录组数据进行差异表达分析.利用ProtScale工具、SinnalP-5.0在线网站、NetPhos-3.1、SOPMA、SMART、STRING数据库对SLC30A5序列特征、相关蛋白和系统进化树进行分析.SLC30A5基因在肝脏组织癌变时表达量显著下调,SLC30A5蛋白属于亲水性蛋白质,含有丰富磷酸化位点,没有信号肽,属于膜转运蛋白.SLC30A5蛋白和SLC30A6蛋白共同作用可以影响肝癌的发生发展.SLC30A5蛋白可能是肝癌新的预后生物标志物,为肝癌治疗手段提供新方向.Sequence characterisation of zinc metabolism related genes in hepatocellular carcinoma,solute carrier family 30 member5(SLC30A5),was analysed using bioinformatics techniques.Transcriptome data of hepatocellular carcinoma,gastric carcinoma,pancreatic carcinoma and normal liver tissues were downloaded from the GEO database for differential expression analysis.Sequence characterisation,related proteins and phylogenetic tree of SLC30A5 were analysed using ProtScale tool,SinnalP-5.0 online website,NetPhos-3.1,SOPMA,SMART and STRING databases.The expression of SLC30A5 gene was significantly down-regulated in cancerous liver tissues,and the protein of SLC30A5 belongs to the group of hydrophilic SLC30A5 protein is a hydrophilic protein with abundant phosphorylation sites,no signal peptide,and is a membrane transporter protein.SLC30A5 and SLC30A6 proteins work together to influence the development of hepatocellular carcinoma.SLC30A5 protein may be a new prognostic biomarker of hepatocellular carcinoma,which may provide a new direction for hepatocellular carcinoma treatment.

关 键 词：肝癌 锌代谢相关基因 SLC30A5 生物信息学 

分 类 号：Q7[生物学—分子生物学]