乙肝两对半化学发光法联合DNA检验对慢性乙肝诊断、肝功能评估的价值  

The value of two and a half pairs of hepatitis B(chemiluminescence method)combined with DNA test for the diagnosis of chronic hepatitis B and liver function assessment

作　　者：邱艺辉 杨清梅 QIU Yi-hui;YANG Qing-mei(Department of Medical Laboratory,Tong'an Branch Hospital of Xiamen University Affiliated First Hospital(Xiamen Third Hospital),Xiamen 361100,China)

机构地区：[1]厦门大学附属第一医院同安院区(厦门市第三医院)医学检验科,361100

出　　处：《中国现代药物应用》2024年第24期58-62,共5页Chinese Journal of Modern Drug Application

摘　　要：目的 分析乙型肝炎(乙肝)两对半化学发光法联合DNA检验对慢性乙肝诊断、肝功能评估的价值。方法 选取200例接受检查和治疗的慢性乙肝患者为研究对象,根据检测方式的不同分为观察组(n=100)和对照组(n=100)。观察组患者接受乙肝两对半化学发光法联合DNA检测,对照组患者接受单纯性乙肝两对半化学发光法检测。对比两组患者乙肝两对半[乙肝核心抗体(HBc Ab)、乙肝e抗原(HBe Ag)、乙肝e抗体(HBe Ab)、乙肝表面抗原(HBs Ag)、乙肝表面抗体(HBs Ab)]阳性率,分析HBs Ag、HBe Ag与乙肝病毒DNA(HBV-DNA)及肝功能指标的关系,对比HBe Ag阳性患者和HBe Ag阴性患者肝功能指标。结果 观察组的HBc Ab、HBe Ag、HBe Ab、HBs Ag、HBs Ab阳性率分别为72.00%、56.00%、49.00%、69.00%、32.00%,明显高于对照组的51.00%、41.00%、34.00%、55.00%、19.00%,差异有统计学意义(P<0.05)。HBs Ag阳性患者HBV-DNA<10~3 copies/ml占比高于HBs Ag阴性患者,HBV-DNA 10~3~10~5 copies/ml占比低于HBs Ag阴性患者,差异有统计学意义(P<0.05);HBs Ag阳性与HBs Ag阴性患者丙氨酸氨基转移酵(ALT)>40 U/L、天门冬氨酸氨基转移酶(AST)>45 U/L占比对比无显著差异(P>0.05)。HBe Ag阳性患者HBV-DNA<10~3 copies/ml占比、AST>45 U/L占比高于HBe Ag阴性患者,HBV-DNA>10~5 copies/ml占比低于HBe Ag阴性患者,差异有统计学意义(P<0.05);HBs Ag阳性与HBe Ag阴性患者ALT>40 U/L占比对比无显著差异(P>0.05)。HBe Ag阳性患者ALT(70.24±3.52)U/L、碱性磷酸酶(ALP)(123.52±11.86)U/L、直接胆红素(DBil)(10.68±1.57)μmol/L、总胆红素(TBil)(23.49±2.39)μmol/L、AST(88.65±4.95)U/L、γ-谷氨酰转移酶(γ-GT)(101.37±14.53)U/L均明显高于HBe Ag阴性患者的(59.37±2.16)U/L、(101.64±8.73)U/L、(7.83±0.85)μmol/L、(18.67±1.76)μmol/L、(62.76±3.27)U/L、(86.72±7.64)U/L,血清白蛋白(ALB)(51.35±2.33)g/L明显低于HBe Ag阴性患者的(57.96±3.41)g/L,差异有统计学意义(P<0.05)。结论 单纯性化Objective To analyze the value of two and a half pairs of hepatitis B(chemiluminescence method)combined with DNA test for the diagnosis of chronic hepatitis B and liver function assessment.Methods A total of 200 patients with chronic hepatitis B who underwent examination and treatment were selected as the subjects of this study,and were divided into an observation group(n=100)and a control group(n=100)according to different test methods.The patients in the observation group were received two and half pairs of hepatitis B(chemiluminescence method)combined with DNA test,and the patients in the control group received two and a half pairs of hepatitis B(chemiluminescence method)test.The positive rate of two and a half pairs of hepatitis B[hepatitis B core antibody(HBcAb),hepatitis B e antigen(HBeAg),Hepatitis B e antibody(HBeAb),hepatitis B surface antigen(HBsAg),hepatitis B surface antibody(HBsAb)]were compared.The correlation of HBsAg and HBeAg with hepatitis B virus DNA(HBV-DNA)and liver function indexes was analyzed,and the liver function indexes of HBeAg-positive patients and HBeAg-negative patients were compared.Results The positive rates of HBcAb,HBeAg,HBeAb,HBsAg,HBsAb in the observation group were 72.00%,56.00%,49.00%,69.00%,and 32.00%,which were significantly higher than 51.00%,41.00%,34.00%,55.00%,and 19.00%in the control group,and the differences were statistically significant(P<0.05).3 The percentage of HBV-DNA<10 copies/ml in HBsAg-positive patients was higher than that in HBsAg-negative patients,and the percentage of HBV-DNA 103-105 copies/ml was lower than that in HBsAg-negative patients,and the difference was statistically significant(P<0.05).There was no significant difference in the comparison of the percentage of alanine aminotrasferase(ALT)>40 U/L and aspartate transaminase(AST)>45 U/L between HBsAg-positive and HBsAg-negative patients(P>0.05).The percentage of HBV-DNA<103×copies/ml and the percentage of AST>45 U/L in HBeAg-positive 5 patients were higher than those in HBeAg-negative patients,an

关 键 词：化学发光法 DNA检测 乙型肝炎两对半检测 慢性乙型肝炎 

分 类 号：I20[文学—中国文学]